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Literature summary for 3.2.1.21 extracted from

  • Belancic, A.; Gunata, Z.; Vallier, M.J.; Agosin, E.
    Beta-glucosidase from the grape native yeast Debaryomyces vanrijiae: purification, characterization, and its effect on monoterpene content of a Muscat grape juice (2003), J. Agric. Food Chem., 51, 1453-1459.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 48% inhibition Schwanniomyces vanrijiae
D-glucono-delta-lactone
-
 Schwanniomyces vanrijiae
D-glucose
-
 Schwanniomyces vanrijiae
ethanol 10-15%, 20-40% inhibition, complete inhibition above 30% Schwanniomyces vanrijiae
Sodium dodecyl sulfate 0.4%, complete inhibition Schwanniomyces vanrijiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
 p-nitro-phenyl-beta-D-glucopyranoside 40°C, pH 5.0 Schwanniomyces vanrijiae
1.07
-
 geranyl-beta-D-glucopyranoside 40°C, pH 5.0 Schwanniomyces vanrijiae
57.9
-
 cellobiose 40°C, pH 5.0 Schwanniomyces vanrijiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, SDS-PAGE Schwanniomyces vanrijiae

Organism

Organism UniProt Comment Textmining
Schwanniomyces vanrijiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Schwanniomyces vanrijiae

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, Ultrogel, DEAE-Sepharose, chromatofocusing Schwanniomyces vanrijiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1278
-
-
 Schwanniomyces vanrijiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl-beta-D-glucopyranoside + H2O
-
 Schwanniomyces vanrijiae benzyl alcohol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Schwanniomyces vanrijiae 2 beta-D-glucose
-
 ?
geranyl-beta-D-glucopyranoside + H2O
-
 Schwanniomyces vanrijiae geraniol + beta-D-glucose
-
 ?
linalool-beta-D-glucopyranoside + H2O
-
 Schwanniomyces vanrijiae linalool + beta-D-glucose
-
 ?
nerol-beta-D-glucopyranoside + H2O
-
 Schwanniomyces vanrijiae nerol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Schwanniomyces vanrijiae p-nitrophenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Schwanniomyces vanrijiae

Synonyms

Synonyms Comment Organism
DV-BG
-
 Schwanniomyces vanrijiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Schwanniomyces vanrijiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 approx. 40% of maximal activity at 20°C, approx. 205 of maximal activity at 60°C Schwanniomyces vanrijiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 no loss of activity after 1 h Schwanniomyces vanrijiae
50
-
 approx. 40% loss of activity after 1 h Schwanniomyces vanrijiae
60
-
 approx. 60% loss of activity after 1 h Schwanniomyces vanrijiae
70
-
 complete loss of activity after 1 h Schwanniomyces vanrijiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Schwanniomyces vanrijiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.15
-
 approx. 80% loss of activity after 24 h Schwanniomyces vanrijiae
4 5.5 approx. 50% loss of activity after 24 h Schwanniomyces vanrijiae
7
-
 approx. 40% loss of activity after 24 h Schwanniomyces vanrijiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
 D-glucono-delta-lactone 40°C, pH 5.0 Schwanniomyces vanrijiae
439
-
 D-glucose 40°C, pH 5.0 Schwanniomyces vanrijiae

pI Value

Organism Comment pI Value Maximum pI Value
Schwanniomyces vanrijiae chromatofocusing, narrow-range isoelectric focusing
-
 3