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Literature summary for 3.2.1.21 extracted from
- beta-Glucosidases from five black Aspergillus species: Study of their physico-chemical and biocatalytic properties (2000), J. Agric. Food Chem., 48, 4929-4936.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | - |
Aspergillus aculeatus |  |
| D-glucose | - |
Aspergillus foetidus | |
| D-glucose | - |
Aspergillus japonicus | |
| D-glucose | - |
Aspergillus niger | |
| D-glucose | - |
Aspergillus tubingensis | |
| p-nitrophenyl-beta-D-glucopyranoside | strong substrate inhibition above 0.5 mM | Aspergillus aculeatus | |
| p-nitrophenyl-beta-D-glucopyranoside | minimal substrate inhibition above 1.5 mM | Aspergillus foetidus | |
| p-nitrophenyl-beta-D-glucopyranoside | minimal substrate inhibition above 1.5 mM | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, 5.0 | Aspergillus japonicus | |
| 0.23 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 4.6 | Aspergillus aculeatus | |
| 0.41 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 4.6 | Aspergillus foetidus | |
| 0.59 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 4.6 | Aspergillus tubingensis | |
| 0.64 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 4.6 | Aspergillus niger | |
| 1.16 | - |
cellobiose | 30°C, 5.0 | Aspergillus japonicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
x * 93000, SDS-PAGE | Aspergillus aculeatus |
| 111000 | - |
x * 111000, SDS-PAGE | Aspergillus tubingensis |
| 121000 | - |
x * 121000, SDS-PAGE | Aspergillus japonicus |
| 130000 | - |
x * 130000, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | - |
- |
- |
| Aspergillus foetidus | - |
- |
- |
| Aspergillus japonicus | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus tubingensis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus niger |
| glycoprotein | - |
Aspergillus japonicus |
| glycoprotein | - |
Aspergillus foetidus |
| glycoprotein | - |
Aspergillus tubingensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
- |
Aspergillus japonicus |
- |
Aspergillus foetidus |
- |
Aspergillus tubingensis |
- |
Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phenylethyl-beta-D-glucopyranoside + H2O | 51% of activity with p-nitrophenyl-beta-D-glucopyranoside | Aspergillus japonicus | 2-phenylethanol + beta-D-glucose | - |
? | |
| benzyl-beta-D-glucopyranoside + H2O | 17% of activity with p-nitrophenyl-beta-d-glucopyranoside | Aspergillus japonicus | benzyl alcohol + beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Aspergillus tubingensis | 2 beta-D-glucose | - |
? | |
| cellobiose + H2O | 15-30% of activity with p-nitrophenyl-beta-d-glucopyranoside | Aspergillus japonicus | 2 beta-D-glucose | - |
? | |
| citronellyl-beta-D-glucopyranoside + H2O | 61.4% of activity with p-nitrophenyl-beta-D-glucopyranoside | Aspergillus japonicus | citronellol + beta-D-glucose | - |
? | |
| dodecyl-beta-D-glucopyranoside + H2O | 15-30% of activity with p-nitrophenyl-beta-d-glucopyranoside | Aspergillus japonicus | dodecanol + beta-D-glucose | - |
? | |
| gentiobiose + H2O | 91% of activity with p-nitrophenyl-beta-D-glucopyranoside | Aspergillus japonicus | 2 beta-D-glucose | - |
? | |
| geranyl-beta-D-glucopyranoside + H2O | 62.7% of activity with p-nitrophenyl-beta-D-glucopyranoside | Aspergillus japonicus | geraniol + beta-D-glucose | - |
? | |
| hexyl-beta-D-glucopyranoside + H2O | 15% of activity with p-nitrophenyl-beta-d-glucopyranoside | Aspergillus japonicus | hexanol + beta-D-glucose | - |
? | |
| laminaribiose + H2O | 67% of activity with p-nitrophenyl-beta-D-glucopyranoside | Aspergillus japonicus | 2 beta-D-glucose | - |
? | |
| octyl-beta-D-glucopyranoside + H2O | 15% of activity with p-nitrophenyl-beta-d-glucopyranoside | Aspergillus japonicus | octanol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Aspergillus niger | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Aspergillus japonicus | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Aspergillus foetidus | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Aspergillus tubingensis | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Aspergillus aculeatus | p-nitrophenol + beta-D-glucose | - |
? | |
| sophorose + H2O | 15-30% of activity with p-nitrophenyl-beta-d-glucopyranoside | Aspergillus japonicus | 2 beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 130000, SDS-PAGE | Aspergillus niger |
| ? | x * 93000, SDS-PAGE | Aspergillus aculeatus |
| ? | x * 111000, SDS-PAGE | Aspergillus tubingensis |
| ? | x * 120000-142000, SDS-PAGE | Aspergillus foetidus |
| ? | x * 121000, SDS-PAGE | Aspergillus japonicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| A1 | - |
Aspergillus niger |
| A1 | - |
Aspergillus aculeatus |
| F1 | - |
Aspergillus foetidus |
| J1 | - |
Aspergillus japonicus |
| T1 | - |
Aspergillus tubingensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus niger |
| 65 | - |
- |
Aspergillus japonicus |
| 65 | - |
- |
Aspergillus foetidus |
| 65 | - |
- |
Aspergillus tubingensis |
| 65 | - |
- |
Aspergillus aculeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Aspergillus tubingensis |
| 4.8 | - |
- |
Aspergillus niger |
| 4.8 | - |
- |
Aspergillus foetidus |
| 5 | - |
- |
Aspergillus aculeatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | approx. 20% of maximal activity at pH 3.0, approx. 10% of maximal activity at pH 7.0 | Aspergillus japonicus |
| 3 | 7 | approx. 20% of maximal activity at pH 3.0, approx. 10% of maximal activity at pH 7.0 | Aspergillus tubingensis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
D-glucose | 30°C, pH 5.0 | Aspergillus niger | |
| 3.7 | - |
D-glucose | 30°C, pH 5.0 | Aspergillus aculeatus | |
| 5.8 | - |
D-glucose | 30°C, pH 4.6 | Aspergillus tubingensis | |
| 8.1 | - |
D-glucose | 30°C, pH 5.0 | Aspergillus foetidus | |
| 9.2 | - |
D-glucose | 30°C, 5.0 | Aspergillus japonicus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
4.2 |
| Aspergillus foetidus | isoelectric focusing | - |
4.2 |
| Aspergillus tubingensis | isoelectric focusing | - |
4.2 |
| Aspergillus japonicus | isoelectric focusing | - |
4.7 |
| Aspergillus aculeatus | isoelectric focusing | - |
4.7 |
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