Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dimethylsulfoxide | 2 mM, 23% activation | Sclerotinia sclerotiorum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 90% inhibition | Sclerotinia sclerotiorum | |
Fe2+ | 5 mM, 97% inhibition | Sclerotinia sclerotiorum | |
Zn2+ | 5 mM, 43% inhibition | Sclerotinia sclerotiorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
p-nitrophenyl-beta-D-glucopyranoside | 60°C, pH 5.0 | Sclerotinia sclerotiorum | |
2.8 | - |
cellobiose | 60°C, pH 5.0 | Sclerotinia sclerotiorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 5 mM, 21% activation | Sclerotinia sclerotiorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96500 | - |
2 * 96500, SDS-PAGE | Sclerotinia sclerotiorum |
196000 | - |
gel filtration | Sclerotinia sclerotiorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Sclerotinia sclerotiorum | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sclerotinia sclerotiorum | - |
filamentous fungi | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, TSK-DEAE-5PW, TSK 3000 SW | Sclerotinia sclerotiorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
277.5 | - |
- |
Sclerotinia sclerotiorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | - |
Sclerotinia sclerotiorum | 2 beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Sclerotinia sclerotiorum | p-nitrophenol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 96500, SDS-PAGE | Sclerotinia sclerotiorum |
Synonyms | Comment | Organism |
---|---|---|
beta-glu1 | - |
Sclerotinia sclerotiorum |
beta-glu2 | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Sclerotinia sclerotiorum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50% loss of activity after 30 min | Sclerotinia sclerotiorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
at 60°C | Sclerotinia sclerotiorum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | no loss of activity | Sclerotinia sclerotiorum |