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Literature summary for 3.2.1.21 extracted from

  • Lusis, A.J.; Becker, R.R.
    The beta-glucosidase system of the thermophilic fungus Chaetomium thermophile var. coprophile n. var. (1973), Biochim. Biophys. Acta, 329, 5-16.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose Ki for cellobiase activity: 91 mM, Ki for cell-bound aryl-beta-glucosidase activity: 0.53 mM, Ki for extracellular aryl-beta-glucosidase activity: 0.34 mM Thermochaetoides thermophila
cellobiose Ki for cellobiase activity: 0.31 mM, Ki for cell-bound aryl-beta-glucosidase activity: 0.72 mM, Ki for extracellular aryl-beta-glucosidase activity: 0.56 mM Thermochaetoides thermophila
p-chloromercuribenzoic acid sensitivity only for cellobiase activity Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 4-nitrophenyl-beta-D-glucopyranoside for extracellular aryl-beta-D-glucosidase Thermochaetoides thermophila
0.089
-
 4-nitrophenyl-beta-D-glucopyranoside for cell-bound aryl-beta-D-glucosidase Thermochaetoides thermophila
1.1
-
 4-nitrophenyl-beta-D-glucopyranoside cellobiase Thermochaetoides thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
cell associated one cellobiase and one aryl-beta-glucosidase Thermochaetoides thermophila
-
-
extracellular aryl-beta-glucosidase Thermochaetoides thermophila
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermochaetoides thermophila cellobiose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermochaetoides thermophila no activity for aryl-beta-D-glucosidases beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
 var. coprophile n. var.
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture 3 different forms, one with cellobiose-activity, two with exclusive aryl-beta-glucosidase activity Thermochaetoides thermophila
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermochaetoides thermophila 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermochaetoides thermophila beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O no activity for aryl-beta-D-glucosidases Thermochaetoides thermophila beta-D-glucose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
aryl-beta-D-glucosidase
-
 Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54
-
 completely stable cell-bound cellobiase, cell-bound aryl-beta-glucosidase loses 50% of its activity in 5 h Thermochaetoides thermophila