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Literature summary for 3.2.1.21 extracted from

  • Sano, K.; Amemura, A.; Harada, T.
    Purification and properties of a beta-1,6-glucosidase from Flavobacterium (1975), Biochim. Biophys. Acta, 377, 410-420.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition at 2 mM Flavobacterium sp.
beta-D-glucose
-
 Flavobacterium sp.
Cu2+ strong inhibition at 2 mM Flavobacterium sp.
D-glucono-1,5-lactone
-
 Flavobacterium sp.
Fe3+ strong inhibition at 2 mM Flavobacterium sp.
Hg2+ strong inhibition at 2 mM Flavobacterium sp.
N-ethylmaleimide
-
 Flavobacterium sp.
p-chloromercuribenzoic acid
-
 Flavobacterium sp.
Tris inhibitory effect Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 luteose in mg/ml Flavobacterium sp.
0.28
-
 gentiobiose
-
 Flavobacterium sp.
0.3
-
 Gentiotriose
-
 Flavobacterium sp.
0.42
-
 Gentiotetraose
-
 Flavobacterium sp.
0.46
-
 Gentiopentaose
-
 Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Flavobacterium sp. 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration, SDS-PAGE Flavobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Flavobacterium sp. very low activity beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Flavobacterium sp. laminaribiose beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Flavobacterium sp. M64 very low activity beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Flavobacterium sp. M64 laminaribiose beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Flavobacterium sp. gentiobiose beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O Flavobacterium sp. transglycosylation reaction beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O Flavobacterium sp. M64 gentiobiose beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O Flavobacterium sp. M64 transglycosylation reaction beta-D-glucose + D-glucose
-
 r
gentiopentaose + 4 H2O Flavobacterium sp. specific for beta-1,6-linkages, activity decreases with number of glucose residues 5 D-glucose
-
 ?
gentiotetraose + 3 H2O Flavobacterium sp.
-
 4 D-glucose
-
 ?
gentiotriose + 2 H2O Flavobacterium sp.
-
 3 D-glucose
-
 ?
luteose + H2O Flavobacterium sp. very low activity beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 M64
-
Flavobacterium sp. M64
-
 M64
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65.4
-
-
 Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O very low activity Flavobacterium sp. beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Flavobacterium sp. beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O very low activity Flavobacterium sp. M64 beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Flavobacterium sp. M64 beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Flavobacterium sp. 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Flavobacterium sp. M64 4-nitrophenol + D-glucopyranose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Flavobacterium sp. beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O transglycosylation reaction Flavobacterium sp. beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Flavobacterium sp. M64 beta-D-glucose + D-glucose
-
 r
6-O-beta-D-glucopyranosyl-D-glucose + H2O transglycosylation reaction Flavobacterium sp. M64 beta-D-glucose + D-glucose
-
 r
gentiobiose + H2O
-
 Flavobacterium sp. 2 beta-D-glucose
-
 ?
gentiopentaose + 4 H2O specific for beta-1,6-linkages, activity decreases with number of glucose residues Flavobacterium sp. 5 D-glucose
-
 ?
gentiotetraose + 3 H2O
-
 Flavobacterium sp. 4 D-glucose
-
 ?
gentiotriose + 2 H2O
-
 Flavobacterium sp. 3 D-glucose
-
 ?
laminaran + H2O low activity Flavobacterium sp. beta-D-glucose + ?
-
 ?
luteose + H2O very low activity Flavobacterium sp. beta-D-glucose + ?
-
 ?
methyl-beta-D-glucoside + H2O very low activity Flavobacterium sp. beta-D-glucose + methanol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Flavobacterium sp.