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Literature summary for 3.2.1.21 extracted from

  • Herr, D.; Baumer, F.; Dellweg, H.
    Purification and properties of an extracellular beta-glucosidase from Lenzites trabea (1978), Eur. J. Appl. Microbiol. Biotechnol., 5, 29-36.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose non-competitive inhibition, Ki: 2.7 mM Lenzites trabea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 4-nitrophenyl-beta-D-cellobioside
-
 Lenzites trabea
0.41
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Lenzites trabea
0.69
-
 2-nitrophenyl-beta-D-xylopyranoside
-
 Lenzites trabea
1.64
-
 cellobiose
-
 Lenzites trabea
3.22
-
 4-nitrophenyl beta-D-fucopyranoside
-
 Lenzites trabea
3.32
-
 4-nitrophenyl beta-D-xylopyranoside
-
 Lenzites trabea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Lenzites trabea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
 gel filtration Lenzites trabea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Lenzites trabea cellobiose beta-D-glucose + D-glucose
-
 ?
cellopentaose + 4 H2O Lenzites trabea
-
 5 D-glucose
-
 ?
cellotetraose + 3 H2O Lenzites trabea
-
 4 D-glucose
-
 ?
cellotriose + 2 H2O Lenzites trabea
-
 3 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Lenzites trabea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Lenzites trabea

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Lenzites trabea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8500
-
-
 Lenzites trabea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Lenzites trabea 2-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-cellobioside + 2 H2O
-
 Lenzites trabea 4-nitrophenol + 2 beta-D-glucose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Lenzites trabea 4-nitrophenol + beta-D-fucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lenzites trabea 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Lenzites trabea 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Lenzites trabea beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Lenzites trabea 2 beta-D-glucose
-
 ?
cellopentaose + 4 H2O
-
 Lenzites trabea 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Lenzites trabea 4 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Lenzites trabea 3 D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Lenzites trabea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Lenzites trabea