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Literature summary for 3.2.1.21 extracted from
- Physical properties, substrate specificities and a probable mechanism for a beta-D-glucosidase (cellobiase) from midgut cells of the cassava hornworm (Erinnyis ello) (1985), Biochim. Biophys. Acta, 831, 179-185.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucono-1,5-lactone | competitive inhibition, Ki: 0.00085 mM | Erinnyis ello |  |
| gamma-galactonolactone | competitive inhibition, Ki: 0.16 mM | Erinnyis ello | |
| Tris | competitive inhibition, Ki: 0.54 mM | Erinnyis ello | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Erinnyis ello | |
| 0.33 | - |
4-nitrophenyl-beta-D-galactopyranoside | - |
Erinnyis ello | |
| 0.33 | - |
4-nitrophenyl-beta-D-fucoside | - |
Erinnyis ello | |
| 0.63 | - |
2-Nitrophenyl-beta-D-glucopyranoside | - |
Erinnyis ello | |
| 1 | - |
4-nitrophenyl-beta-D-xyloside | - |
Erinnyis ello | |
| 2 | - |
cellobiose | - |
Erinnyis ello | |
| 6.3 | - |
Salicin | - |
Erinnyis ello | |
| 7.4 | - |
2-nitrophenyl-beta-D-galactopyranoside | - |
Erinnyis ello | |
| 17 | - |
lactose | - |
Erinnyis ello | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 129000 | - |
density gradient centrifugation, isoelectric focusing | Erinnyis ello |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | Erinnyis ello | lactose | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Erinnyis ello | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erinnyis ello | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | from larvae | Erinnyis ello | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Erinnyis ello | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Erinnyis ello | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 2-nitrophenyl beta-D-glucopyranoside + H2O | retention of configuration | Erinnyis ello | 2-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-fucoside + H2O | - |
Erinnyis ello | 4-nitrophenol + beta-D-fucose | - |
? | |
| 4-nitrophenyl beta-D-galactoside + H2O | - |
Erinnyis ello | 4-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Erinnyis ello | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Erinnyis ello | 4-nitrophenol + beta-D-xylose | - |
? | |
| 4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Erinnyis ello | beta-D-galactose + D-glucose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Erinnyis ello | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Erinnyis ello | 2 beta-D-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
inactivation above | Erinnyis ello |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Erinnyis ello |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
stable for 2 h at 15-40°C | Erinnyis ello |
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