KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermus sp. | |
2 | - |
cellobiose | - |
Thermus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | induction of enzyme synthesis by addition of cellobiose or laminaribiose to the medium | Thermus sp. | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Thermus sp. | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | Thermus sp. | low activity | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | Thermus sp. | lactose | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermus sp. | cellobiose | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermus sp. | low activity | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermus sp. | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
cellohexaose + 5 H2O | Thermus sp. | low activity | 6 D-glucose | - |
? | |
cellopentaose + 4 H2O | Thermus sp. | - |
5 D-glucose | - |
? | |
cellotetraose + 3 H2O | Thermus sp. | - |
4 D-glucose | - |
? | |
cellotriose + 2 H2O | Thermus sp. | - |
3 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Thermus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Thermus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.34 | - |
in cell-free extract for laminaribiose, maximum rate of all substrates tested | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Thermus sp. | beta-D-glucose + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus sp. | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | low activity | Thermus sp. | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Thermus sp. | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermus sp. | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Thermus sp. | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Thermus sp. | beta-D-glucose + D-glucose | - |
? | |
cellobiose + H2O | - |
Thermus sp. | 2 beta-D-glucose | - |
? | |
cellohexaose + 5 H2O | low activity | Thermus sp. | 6 D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
Thermus sp. | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Thermus sp. | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Thermus sp. | 3 D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life: 5 days | Thermus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | optimal activity within | Thermus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | stable for 2 h at 70°C | Thermus sp. |