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Literature summary for 3.2.1.21 extracted from

  • Takase, M.; Horikoshi, K.
    A thermostable beta-glucosidase isolated from a bacterial species of the genus Thermus (1988), Appl. Microbiol. Biotechnol., 29, 55-60.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
4-nitrophenyl-beta-D-glucopyranoside
-
Thermus sp.
2
-
cellobiose
-
Thermus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular induction of enzyme synthesis by addition of cellobiose or laminaribiose to the medium Thermus sp. 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus sp. laminaribiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus sp. low activity beta-D-galactose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus sp. lactose beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. gentiobiose beta-D-glucose + D-glucose
-
?
cellohexaose + 5 H2O Thermus sp. low activity 6 D-glucose
-
?
cellopentaose + 4 H2O Thermus sp.
-
5 D-glucose
-
?
cellotetraose + 3 H2O Thermus sp.
-
4 D-glucose
-
?
cellotriose + 2 H2O Thermus sp.
-
3 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Thermus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.34
-
in cell-free extract for laminaribiose, maximum rate of all substrates tested Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Thermus sp. beta-D-glucose + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermus sp. 4-nitrophenol + D-glucopyranose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O low activity Thermus sp. beta-D-galactose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Thermus sp. beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermus sp. beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Thermus sp. beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Thermus sp. beta-D-glucose + D-glucose
-
?
cellobiose + H2O
-
Thermus sp. 2 beta-D-glucose
-
?
cellohexaose + 5 H2O low activity Thermus sp. 6 D-glucose
-
?
cellopentaose + 4 H2O
-
Thermus sp. 5 D-glucose
-
?
cellotetraose + 3 H2O
-
Thermus sp. 4 D-glucose
-
?
cellotriose + 2 H2O
-
Thermus sp. 3 D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
half-life: 5 days Thermus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 optimal activity within Thermus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 stable for 2 h at 70°C Thermus sp.