Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucono-1,5-lactone | competitive inhibition, Ki: 0.0004 mM for enzyme A, Ki: 0.0015 mM for enzyme B, Ki: 0.12 mM for cell wall bound enzyme | Sporotrichum pulverulentum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
4-nitrophenyl-beta-D-glucopyranoside | cell wall bound enzyme | Sporotrichum pulverulentum | |
0.15 | - |
4-nitrophenyl-beta-D-glucopyranoside | for enzyme A | Sporotrichum pulverulentum | |
0.21 | - |
4-nitrophenyl-beta-D-glucopyranoside | for enzyme B | Sporotrichum pulverulentum | |
3.7 | - |
cellobiose | for enzyme B | Sporotrichum pulverulentum | |
4.5 | - |
cellobiose | for enzyme A | Sporotrichum pulverulentum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | high extracellular activities, if cellulose serves as carbon source | Sporotrichum pulverulentum | - |
- |
membrane | cell bound activity if cellobiose dominating in the medium | Sporotrichum pulverulentum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
165000 | 182000 | gel filtration, five different forms in culture filtrate | Sporotrichum pulverulentum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Sporotrichum pulverulentum | cellobiose | beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporotrichum pulverulentum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, five different extracellular forms, chromatography steps | Sporotrichum pulverulentum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | five different forms in culture filtrate, 1 form cell-wall bound | Sporotrichum pulverulentum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79 | 102 | 5 different forms from culture filtrate | Sporotrichum pulverulentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sporotrichum pulverulentum | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Sporotrichum pulverulentum | beta-D-glucose + D-glucose | - |
? | |
cellobiose + H2O | - |
Sporotrichum pulverulentum | 2 beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,4-beta-glucosidase | - |
Sporotrichum pulverulentum |