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Literature summary for 3.2.1.21 extracted from

  • Hayashida, S.; Ohta, K.; Mo, K.
    Cellulases of Humicola insolens and Humicola grisea (1988), Methods Enzymol., 160, 323-332.
No PubMed abstract available
Search for more literature for 3.2.1.21

General Stability

General Stability Organism
stable in the presence of urea Humicola insolens
stable in the presence of urea Trichocladium griseum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 55% of original activity inhibitited Humicola insolens
Cu2+ 55% of original activity inhibitited Trichocladium griseum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Humicola insolens
-
-
extracellular
-
 Trichocladium griseum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 SDS-PAGE Humicola insolens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Humicola insolens cellobiose beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Trichocladium griseum cellobiose beta-D-glucose + D-glucose
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide significantly resistant even at high concentrations Humicola insolens
dimethyl sulfoxide significantly resistant even at high concentrations Trichocladium griseum
Ethanol significantly resistant even at high concentrations Humicola insolens
Ethanol significantly resistant even at high concentrations Trichocladium griseum

Organism

Organism UniProt Comment Textmining
Humicola insolens
-
-
-
Trichocladium griseum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichocladium griseum
glycoprotein 1.7%, w/w, mannose, 0.8%, w/w, glucose residues Humicola insolens

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 3step chromatography Humicola insolens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Humicola insolens
-
cell culture
-
 Trichocladium griseum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
266.9
-
-
 Humicola insolens
266.9
-
-
 Trichocladium griseum

Storage Stability

Storage Stability Organism
0-4°C, 0.05 M acetate buffer, pH 5.0, 2 weeks Humicola insolens
0-4°C, 0.05 M acetate buffer, pH 5.0, 2 weeks Trichocladium griseum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Humicola insolens 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Trichocladium griseum 2-hydroxymethylphenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Humicola insolens 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Trichocladium griseum 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Humicola insolens beta-D-glucose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Trichocladium griseum beta-D-glucose + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Humicola insolens
50
-
-
 Trichocladium griseum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for 5 min Humicola insolens
60
-
 stable for 5 min Trichocladium griseum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Humicola insolens
5
-
-
 Trichocladium griseum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable within Humicola insolens
4 8 stable within Trichocladium griseum