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Literature summary for 3.2.1.21 extracted from

  • McCleary, B.V.; Harrington, J.
    Purification of beta-D-glucosidase from Aspergillus niger (1988), Methods Enzymol., 160, 575-583.
No PubMed abstract available

General Stability

General Stability Organism
stable to repeated freezing and thawing Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
cellotriose
-
Aspergillus niger
0.51
-
cellotetraose
-
Aspergillus niger
0.8
-
4-nitrophenyl-beta-D-glucopyranoside
-
Aspergillus niger
1.88
-
gentiobiose
-
Aspergillus niger
1.89
-
cellobiose
-
Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1-4)-beta-D-glucosyl-D-mannobiose + H2O Aspergillus niger
-
beta-D-glucose + D-mannobiose
-
?
(1-4)-beta-D-glucosyl-D-mannose + H2O Aspergillus niger
-
beta-D-glucose + D-mannose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Aspergillus niger sophorose beta-D-glucose + D-glucose
-
?
3(2)-beta-D-glucosyl-D-cellobiose + H2O Aspergillus niger
-
beta-D-glucose + D-cellobiose
-
?
3-O-beta-cellobiosyl-D-glucose + H2O Aspergillus niger
-
beta-D-glucose + D-cellobiose
-
?
3-O-beta-cellotriosyl-D-glucose + H2O Aspergillus niger
-
beta-D-glucose + D-cellotriose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus niger cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus niger gentiobiose beta-D-glucose + D-glucose
-
?
cellopentaose + 4 H2O Aspergillus niger
-
5 D-glucose
-
?
cellotetraose + 3 H2O Aspergillus niger
-
4 D-glucose
-
?
cellotriose + 2 H2O Aspergillus niger
-
3 D-glucose
-
?
gentiotriose + 2 H2O Aspergillus niger
-
3 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
further purification of commercially available enzyme preparation, to homogeneity, chromatography steps Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Novozym 188 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.8
-
-
Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1-4)-beta-D-glucosyl-D-mannobiose + H2O
-
Aspergillus niger beta-D-glucose + D-mannobiose
-
?
(1-4)-beta-D-glucosyl-D-mannose + H2O
-
Aspergillus niger beta-D-glucose + D-mannose
-
?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Aspergillus niger 2-hydroxymethylphenol + beta-D-glucose
-
?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Aspergillus niger 4-hydroxyphenol + beta-D-glucopyranose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O very low activity Aspergillus niger 2-nitrophenol + beta-D-galactopyranose
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O very low activity Aspergillus niger 2-nitrophenol + beta-D-xylopyranose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Aspergillus niger beta-D-glucose + D-glucose
-
?
3(2)-beta-D-glucosyl-D-cellobiose + H2O
-
Aspergillus niger beta-D-glucose + D-cellobiose
-
?
3-O-beta-cellobiosyl-D-glucose + H2O
-
Aspergillus niger beta-D-glucose + D-cellobiose
-
?
3-O-beta-cellotriosyl-D-glucose + H2O
-
Aspergillus niger beta-D-glucose + D-cellotriose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Aspergillus niger 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O very low activity Aspergillus niger 4-nitrophenol + beta-D-mannopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Aspergillus niger 4-nitrophenol + beta-D-xylose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus niger beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Aspergillus niger beta-D-glucose + D-glucose
-
?
cellopentaose + 4 H2O
-
Aspergillus niger 5 D-glucose
-
?
cellotetraose + 3 H2O
-
Aspergillus niger 4 D-glucose
-
?
cellotriose + 2 H2O
-
Aspergillus niger 3 D-glucose
-
?
esculin + H2O
-
Aspergillus niger beta-D-glucose + 6,7-dihydroxycoumarin
-
?
gentiobiose + H2O
-
Aspergillus niger 2 beta-D-glucose
-
?
gentiotriose + 2 H2O
-
Aspergillus niger 3 D-glucose
-
?
methyl-beta-D-glucoside + H2O low activity Aspergillus niger beta-D-glucose + methanol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 75
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
stable up to Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 9 no loss of activity in 2 h at 4°C Aspergillus niger