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Literature summary for 3.2.1.21 extracted from

  • Takase, M.; Horikoshi, K.
    Purification and properties of a beta-glucosidase from Thermus sp. Z-1 (1989), Agric. Biol. Chem., 53, 559-560.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ inactivation at 0.1 mM Thermus sp.
Hg2+ complete inactivation at 0.1 mM Thermus sp.
p-chloromercuribenzoic acid inactivation at 0.1 mM Thermus sp.
SDS inactivation at 0.1 mM Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 4-nitrophenyl-beta-D-mannopyranoside
-
 Thermus sp.
0.21
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Thermus sp.
0.27
-
 4-nitrophenyl-beta-D-fucoside
-
 Thermus sp.
0.6
-
 Salicin
-
 Thermus sp.
2
-
 cellobiose
-
 Thermus sp.
2.8
-
 4-nitrophenyl-beta-D-xyloside
-
 Thermus sp.
6.7
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Thermus sp.
36
-
 lactose
-
 Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration, SDS-PAGE Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus sp. lactose beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus sp. cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Thermus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
 for 4-nitrophenyl-beta-D-mannopyranoside Thermus sp.
59.6
-
 4-nitrophenyl-beta-D-glucopyranoside Thermus sp.
91.5
-
 for 4-nitrophenyl-beta-D-fucoside Thermus sp.
259.9
-
 4-nitrophenyl-beta-D-galactoside Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Thermus sp. 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Thermus sp. 2-hydroxymethylphenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O
-
 Thermus sp. 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Thermus sp. 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermus sp. 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-mannopyranoside + H2O low activity Thermus sp. 4-nitrophenol + beta-D-mannopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Thermus sp. 4-nitrophenol + beta-D-xylose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Thermus sp. beta-D-galactose + D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermus sp. beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Thermus sp. 2 beta-D-glucose
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 85 optimal activity within Thermus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 optimal activity within Thermus sp.