Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paenibacillus polymyxa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucose | competitive inhibition, Ki: 19 mM | Paenibacillus polymyxa | |
DTT | 30% inhibition at 5 mM | Paenibacillus polymyxa | |
Hg2+ | strong inhibition at 1 mM | Paenibacillus polymyxa | |
SDS | 85% inhibition at 5 mM | Paenibacillus polymyxa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Paenibacillus polymyxa | |
13 | - |
cellobiose | - |
Paenibacillus polymyxa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Paenibacillus polymyxa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Paenibacillus polymyxa | cellobiose | beta-D-glucose + D-glucose | additional product: cellotriose by transglycosylation activity | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus polymyxa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Paenibacillus polymyxa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | genomic library | Paenibacillus polymyxa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2417 | - |
- |
Paenibacillus polymyxa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Paenibacillus polymyxa | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Paenibacillus polymyxa | beta-D-glucose + D-glucose | additional product: cellotriose by transglycosylation activity | r | |
cellobiose + H2O | - |
Paenibacillus polymyxa | 2 beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BglA | - |
Paenibacillus polymyxa |