Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | strong inhibition at 10 mM | Millerozyma farinosa | |
beta-D-glucose | competitive inhibition, Ki: 8 mM | Millerozyma farinosa | |
Cu2+ | moderate inhibition at 10 mM | Millerozyma farinosa | |
Fe3+ | moderate inhibition at 10 mM | Millerozyma farinosa | |
Hg2+ | strong inhibition at 10 mM | Millerozyma farinosa | |
Mg2+ | moderate inhibition at 10 mM | Millerozyma farinosa | |
Mn2+ | moderate inhibition at 10 mM | Millerozyma farinosa | |
N-bromosuccinimide | inhibition at 10 mM | Millerozyma farinosa | |
p-chloromercuribenzoic acid | inhibition at 1 mM | Millerozyma farinosa | |
Ru2+ | moderate inhibition at 10 mM | Millerozyma farinosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Millerozyma farinosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | stimulation of enzyme synthesis by cellobiose as carbohydrate source | Millerozyma farinosa | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activation at 10 mM | Millerozyma farinosa | |
Na+ | activation at 10 mM | Millerozyma farinosa | |
Ni2+ | activation at 10 mM | Millerozyma farinosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, SDS-PAGE | Millerozyma farinosa |
220000 | - |
gel filtration | Millerozyma farinosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Millerozyma farinosa | low activity | beta-D-glucose + D-glucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Millerozyma farinosa | sophorose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Millerozyma farinosa | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Millerozyma farinosa | cellobiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Millerozyma farinosa | low activity | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Millerozyma farinosa | low activity | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Millerozyma farinosa | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
alpha-L-arabinofuranose + H2O | Millerozyma farinosa | low activity | alpha-L-arabinofuranose + ? | - |
? | |
alpha-L-rhamnopyranoside + H2O | Millerozyma farinosa | - |
alpha-L-rhamnopyranose + ? | - |
? | |
trehalose + H2O | Millerozyma farinosa | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Millerozyma farinosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2step chromatography | Millerozyma farinosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Millerozyma farinosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.63 | - |
- |
Millerozyma farinosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Millerozyma farinosa | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Millerozyma farinosa | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Millerozyma farinosa | 2-nitrophenol + D-glucopyranose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | low activity | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
4-nitrophenyl beta-D-fucoside + H2O | - |
Millerozyma farinosa | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Millerozyma farinosa | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Millerozyma farinosa | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl-alpha-D-glucoside | - |
Millerozyma farinosa | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Millerozyma farinosa | beta-D-glucose + D-glucose | - |
? | |
alpha-L-arabinofuranose + H2O | low activity | Millerozyma farinosa | alpha-L-arabinofuranose + ? | - |
? | |
alpha-L-rhamnopyranoside + H2O | - |
Millerozyma farinosa | alpha-L-rhamnopyranose + ? | - |
? | |
methyl-beta-D-glucoside + H2O | low activity | Millerozyma farinosa | beta-D-glucose + methanol | - |
? | |
trehalose + H2O | - |
Millerozyma farinosa | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, SDS-PAGE | Millerozyma farinosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Millerozyma farinosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Millerozyma farinosa |