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Literature summary for 3.2.1.21 extracted from

  • Singh, A.; Hayashi, K.
    Construction of chimeric beta-glucosidases with improved enzymatic properties (1995), J. Biol. Chem., 270, 21928-21933.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens
expression in Escherichia coli Cellulomonas gilvus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
4-nitrophenyl-beta-D-xyloside no change for chimeric enzymes Agrobacterium tumefaciens
0.032
-
4-nitrophenyl-beta-D-glucopyranoside marked increase for chimeric enzyme Agrobacterium tumefaciens
0.12
-
4-nitrophenyl-beta-D-fucoside
-
Agrobacterium tumefaciens
1.8
-
4-nitrophenyl-beta-D-glucopyranoside marked decrease for chimeric enzymes Cellulomonas gilvus
6.3
-
4-nitrophenyl-beta-D-xyloside
-
Cellulomonas gilvus
10.8
-
4-nitrophenyl-beta-D-galactoside
-
Cellulomonas gilvus
13.1
-
4-nitrophenyl-beta-D-galactoside
-
Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Agrobacterium tumefaciens cellobiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Cellulomonas gilvus cellobiose beta-D-glucose + D-glucose
-
?
cellohexaose + 5 H2O Agrobacterium tumefaciens marked increase of relative activity for chimeric enzymes 6 D-glucose
-
?
cellohexaose + 5 H2O Cellulomonas gilvus marked increase of relative activity for chimeric enzymes 6 D-glucose
-
?
cellopentaose + 4 H2O Agrobacterium tumefaciens marked increase of relative activity for chimeric enzyme 5 D-glucose
-
?
cellopentaose + 4 H2O Cellulomonas gilvus marked increase of relative activity for chimeric enzyme 5 D-glucose
-
?
cellotetraose + 3 H2O Agrobacterium tumefaciens marked increase of relative activity for chimeric activity 4 D-glucose
-
?
cellotetraose + 3 H2O Cellulomonas gilvus marked increase of relative activity for chimeric activity 4 D-glucose
-
?
cellotriose + 2 H2O Agrobacterium tumefaciens
-
3 D-glucose
-
?
cellotriose + 2 H2O Cellulomonas gilvus
-
3 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Cellulomonas gilvus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Agrobacterium tumefaciens
partial Cellulomonas gilvus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Agrobacterium tumefaciens
-
cell culture
-
Cellulomonas gilvus
-
additional information genomic library Agrobacterium tumefaciens
-
additional information genomic library Cellulomonas gilvus
-
additional information construction of 3 chimeric enzymes derived from substituting segments from the C-terminus of Cellvibrio gilvus and Agrobacterium tumefaciens beta-glucosidase genes Agrobacterium tumefaciens
-
additional information construction of 3 chimeric enzymes derived from substituting segments from the C-terminus of Cellvibrio gilvus and Agrobacterium tumefaciens beta-glucosidase genes Cellulomonas gilvus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucoside + H2O
-
Agrobacterium tumefaciens 4-methylumbelliferone + beta-D-glucose
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
-
Cellulomonas gilvus 4-methylumbelliferone + beta-D-glucose
-
?
4-nitrophenyl beta-D-fucoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-fucoside + H2O
-
Cellulomonas gilvus 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
Cellulomonas gilvus 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Cellulomonas gilvus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Cellulomonas gilvus 4-nitrophenol + beta-D-xylose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Agrobacterium tumefaciens beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Cellulomonas gilvus beta-D-glucose + D-glucose
-
?
cellohexaose + 5 H2O marked increase of relative activity for chimeric enzymes Agrobacterium tumefaciens 6 D-glucose
-
?
cellohexaose + 5 H2O marked increase of relative activity for chimeric enzymes Cellulomonas gilvus 6 D-glucose
-
?
cellopentaose + 4 H2O marked increase of relative activity for chimeric enzyme Agrobacterium tumefaciens 5 D-glucose
-
?
cellopentaose + 4 H2O marked increase of relative activity for chimeric enzyme Cellulomonas gilvus 5 D-glucose
-
?
cellotetraose + 3 H2O marked increase of relative activity for chimeric activity Agrobacterium tumefaciens 4 D-glucose
-
?
cellotetraose + 3 H2O marked increase of relative activity for chimeric activity Cellulomonas gilvus 4 D-glucose
-
?
cellotriose + 2 H2O
-
Agrobacterium tumefaciens 3 D-glucose
-
?
cellotriose + 2 H2O
-
Cellulomonas gilvus 3 D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Cellulomonas gilvus
45 50 for chimeric enzymes Agrobacterium tumefaciens
45 50 for chimeric enzymes Cellulomonas gilvus
60
-
-
Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.4
-
Cellulomonas gilvus
6.6 7 for chimeric enzymes Agrobacterium tumefaciens
6.6 7 for chimeric enzymes Cellulomonas gilvus
7.2 7.4
-
Agrobacterium tumefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 chimeric enzymes, stable within Agrobacterium tumefaciens
4 9 chimeric enzymes, stable within Cellulomonas gilvus
4 8 stable within Cellulomonas gilvus
5 10 stable within Agrobacterium tumefaciens