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Literature summary for 3.2.1.21 extracted from
- A very stable beta-glucosidase from a Candida molischiana mutant strain: enzymatic properties, sequencing, and homology with other yeast beta-glucosidases (1995), Biosci. Biotechnol. Biochem., 59, 1320-1322.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Kuraishia molischiana | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 330000 | - |
native electrophoresis | Kuraishia molischiana |
| 380000 | - |
native electrophoresis, enzyme from mutant strain | Kuraishia molischiana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kuraishia molischiana | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme from mutant strain contains 70%, w/w, carbohydrate, wild-type enzyme 60% carbohydrate, w/w | Kuraishia molischiana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps, wild-type and enzyme from mutant strain | Kuraishia molischiana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Kuraishia molischiana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56 | - |
- |
Kuraishia molischiana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Kuraishia molischiana | 4-nitrophenol + D-glucopyranose | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme from mutant strain | Kuraishia molischiana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme from mutant strain stable up to for 30 min, wild-type enzyme retains 75% of its initial activity | Kuraishia molischiana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
enzyme from mutant strain | Kuraishia molischiana |
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Release 2023.1 (January 2023)
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