Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tris | inhibitory effect | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermus thermophilus | |
0.16 | - |
4-nitrophenyl-beta-D-fucoside | - |
Thermus thermophilus | |
4 | - |
cellobiose | - |
Thermus thermophilus | |
6.6 | - |
4-nitrophenyl-beta-D-galactoside | - |
Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48600 | - |
gel filtration | Thermus thermophilus |
48642 | - |
1 * 48642, deduced from amino acid composition, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Thermus thermophilus | sophorose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Thermus thermophilus | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Thermus thermophilus | preference for beta-1,3-bonds | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | Thermus thermophilus | lactose | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermus thermophilus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermus thermophilus | low activity | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermus thermophilus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
cellopentaose + 4 H2O | Thermus thermophilus | - |
5 D-glucose | - |
? | |
cellotetraose + 3 H2O | Thermus thermophilus | - |
4 D-glucose | - |
? | |
cellotriose + 2 H2O | Thermus thermophilus | - |
3 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Thermus thermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | genomic library | Thermus thermophilus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O | - |
Thermus thermophilus | 2-nitrophenol + beta-D-galactopyranose 6-phosphate | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus thermophilus | 2-nitrophenol + D-glucopyranose | - |
? | |
2-nitrophenyl-beta-D-fucoside + H2O | - |
Thermus thermophilus | 2-nitrophenol + beta-D-fucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Thermus thermophilus | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Thermus thermophilus | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | preference for beta-1,3-bonds | Thermus thermophilus | beta-D-glucose + D-glucose | - |
? | |
4-nitrophenyl beta-D-fucoside + 2-phenylethanol | best substrate for transglycolsylation reaction | Thermus thermophilus | 2-phenylethyl-beta-D-fucoside + 4-nitrophenol | - |
r | |
4-nitrophenyl beta-D-fucoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactoside + 2-phenylethanol | transglycosylation reaction | Thermus thermophilus | 2-phenylethyl beta-D-galactoside + 4-nitrophenol | - |
r | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucoside + 2-phenylethanol | transglycosylation reaction | Thermus thermophilus | 2-phenylethyl-beta-D-glucoside + 4-nitrophenol | - |
r | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Thermus thermophilus | 4-nitrophenol + beta-D-xylose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucose + H2O | lactose | Thermus thermophilus | beta-D-galactose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermus thermophilus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Thermus thermophilus | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Thermus thermophilus | beta-D-glucose + D-glucose | - |
? | |
cellobiose + H2O | - |
Thermus thermophilus | 2 beta-D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
Thermus thermophilus | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Thermus thermophilus | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Thermus thermophilus | 3 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48642, deduced from amino acid composition, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
88 | - |
for 4-nitrophenyl-beta-D-glucoside | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable up to | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Thermus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | retaining more than 80% of the activity | Thermus thermophilus |