Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Dion, M.; Fourage, L.; Hallet, J.N.; Colas, B.
    Cloning and expression of a beta-glycosidase gene from Thermus thermophilus. Sequence and biochemical characterization of the encoded enzyme (1999), Glycoconj. J., 16, 27-37.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Tris inhibitory effect Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
4-nitrophenyl-beta-D-glucopyranoside
-
Thermus thermophilus
0.16
-
4-nitrophenyl-beta-D-fucoside
-
Thermus thermophilus
4
-
cellobiose
-
Thermus thermophilus
6.6
-
4-nitrophenyl-beta-D-galactoside
-
Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48600
-
gel filtration Thermus thermophilus
48642
-
1 * 48642, deduced from amino acid composition, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus thermophilus sophorose beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus thermophilus laminaribiose beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermus thermophilus preference for beta-1,3-bonds beta-D-glucose + D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O Thermus thermophilus lactose beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus thermophilus cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus thermophilus low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Thermus thermophilus gentiobiose beta-D-glucose + D-glucose
-
?
cellopentaose + 4 H2O Thermus thermophilus
-
5 D-glucose
-
?
cellotetraose + 3 H2O Thermus thermophilus
-
4 D-glucose
-
?
cellotriose + 2 H2O Thermus thermophilus
-
3 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Thermus thermophilus

Source Tissue

Source Tissue Comment Organism Textmining
additional information genomic library Thermus thermophilus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermus thermophilus 2-nitrophenol + beta-D-galactopyranose
-
?
2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
-
Thermus thermophilus 2-nitrophenol + beta-D-galactopyranose 6-phosphate
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermus thermophilus 2-nitrophenol + D-glucopyranose
-
?
2-nitrophenyl-beta-D-fucoside + H2O
-
Thermus thermophilus 2-nitrophenol + beta-D-fucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Thermus thermophilus beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Thermus thermophilus beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O preference for beta-1,3-bonds Thermus thermophilus beta-D-glucose + D-glucose
-
?
4-nitrophenyl beta-D-fucoside + 2-phenylethanol best substrate for transglycolsylation reaction Thermus thermophilus 2-phenylethyl-beta-D-fucoside + 4-nitrophenol
-
r
4-nitrophenyl beta-D-fucoside + H2O
-
Thermus thermophilus 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-galactoside + 2-phenylethanol transglycosylation reaction Thermus thermophilus 2-phenylethyl beta-D-galactoside + 4-nitrophenol
-
r
4-nitrophenyl beta-D-galactoside + H2O
-
Thermus thermophilus 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermus thermophilus 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucoside + 2-phenylethanol transglycosylation reaction Thermus thermophilus 2-phenylethyl-beta-D-glucoside + 4-nitrophenol
-
r
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Thermus thermophilus 4-nitrophenol + beta-D-xylose
-
?
4-O-beta-D-galactopyranosyl-D-glucose + H2O lactose Thermus thermophilus beta-D-galactose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermus thermophilus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Thermus thermophilus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Thermus thermophilus beta-D-glucose + D-glucose
-
?
cellobiose + H2O
-
Thermus thermophilus 2 beta-D-glucose
-
?
cellopentaose + 4 H2O
-
Thermus thermophilus 5 D-glucose
-
?
cellotetraose + 3 H2O
-
Thermus thermophilus 4 D-glucose
-
?
cellotriose + 2 H2O
-
Thermus thermophilus 3 D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48642, deduced from amino acid composition, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
88
-
for 4-nitrophenyl-beta-D-glucoside Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
stable up to Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
4-nitrophenyl-beta-D-glucopyranoside
-
Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Thermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 retaining more than 80% of the activity Thermus thermophilus