Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, 2.2 A resolution structure | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5R)-5-fluoro-alpha-D-xylopyranosyl fluoride | mechanism-based inactivator | Escherichia coli | |
(5S)-5-fluoro-alpha-D-xylopyranosyl fluoride | mechanism-based inactivator | Escherichia coli | |
1-deoxynojirimycin | - |
Escherichia coli | |
1-deoxyxylonojirimycin | - |
Escherichia coli | |
xyloisofagomine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
4-nitrophenyl-alpha-D-xyloside | pH 7.0, 37°C | Escherichia coli | |
0.97 | - |
alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C | Escherichia coli | |
7.7 | - |
4-nitrophenyl-alpha-D-glucopyranoside | pH 7.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on hydrolysis of 4-nitrophenyl-alpha-D-xyloside: Ca2+, Mg2+, Mn2+, Zn2+, Ni2+, Cu2+, and Co2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P31434 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, pH 7.0, retains full activity for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | low activity | Escherichia coli | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-nitrophenyl-alpha-D-xyloside + H2O | - |
Escherichia coli | 4-nitrophenol + alpha-D-xylose | - |
? | |
alpha-D-xylopyranosyl fluoride + H2O | - |
Escherichia coli | alpha-D-xylose + fluoride | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | crystallographic data | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
YicI | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
50 | - |
hydrolysis of 4-nitrophenyl-alpha-D-xyloside increases up to 50°C, activity is rapidly lost at higher temperatures | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
7 | - |
37°C, retains full activity for 48 h | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
4-nitrophenyl-alpha-D-glucopyranoside | pH 7.0, 37°C | Escherichia coli | |
0.13 | - |
4-nitrophenyl-alpha-D-xyloside | pH 7.0, 37°C | Escherichia coli | |
75 | - |
alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
hydrolysis of 4-nitrophenyl-alpha-D-xyloside | Escherichia coli |
7 | - |
assay at | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 45% of maximal activity | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
(5R)-5-fluoro-alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C, mechanism-based inactivator | Escherichia coli | |
0.0009 | - |
1-deoxyxylonojirimycin | pH 7.0, 37°C | Escherichia coli | |
0.0098 | - |
(5S)-5-fluoro-alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C, mechanism-based inactivator | Escherichia coli | |
0.13 | - |
1-deoxynojirimycin | pH 7.0, 37°C | Escherichia coli | |
0.17 | - |
xyloisofagomine | pH 7.0, 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
4-nitrophenyl-alpha-D-glucopyranoside | pH 7.0, 37°C | Escherichia coli | |
0.18 | - |
4-nitrophenyl-alpha-D-xyloside | pH 7.0, 37°C | Escherichia coli | |
77.3 | - |
alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C | Escherichia coli |