Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydrophobic affinity ligand L-tryptophan immobilized magnetic poly(glycidyl methacrylate) [m-poly(GMA)] beads in monosize form (0.0016 mM in diameter) are used for the affinity purification of lysozyme from chicken egg white. The chemisorption processes can be the rate-limiting step in the adsorption process. After 10 adsorption-elution cycles, m-poly(GMA)-L-tryptophan beads can be used without significant loss in lysozyme adsorption capacity | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg white | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
one unit lysozyme activity is defined as the amount of enzyme causing a decrase of 0.001 optical density value per minute at 25°C and pH 7.0. Specific activity of the eluted lysozyme (62580 U/mg) is higher than that obtained with a commercially available pure lysozyme Sigma (60000 U/mg) | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidoglycan + H2O | Micrococcus lysodeikticus cells | Gallus gallus | ? | - |
? |