Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Struthio camelus |
Protein Variants | Comment | Organism |
---|---|---|
E73A | no hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose at 50°C for 48 h. Decrease in Tm-value is 3.2°C as compared to wild-type value | Struthio camelus |
E73D | detectable level of activity, but lytic activity against Micrococcus lysodeikticus is drastically reduced as compared to wild-type activity. Decrease in Tm-value is 6.1°C as compared to wild-type value | Struthio camelus |
E73Q | no hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose at 50°C for 48 h. Decrease in Tm-value is 4.0°C as compared to wild-type value | Struthio camelus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Struthio camelus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Struthio camelus |
Renatured (Comment) | Organism |
---|---|
when the thermal denaturation curves are analyzed in the absence of guanidine hydrochloride, the reversibility calculated is 76% for the wild-type enzyme, 80% for E73D, 80% for E73Q and 79% for E73A. In the presence of 0.5 M guanidine hydrochloride, the reversibility of the thermal unfolding for the wild type and mutant proteins is more than 95% | Struthio camelus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | wild-type protein hydrolyzes N,N',N'',N''',N''''-pentaacetylchitopentaose almost completely on 140 min reaction. N,N',N'',N'''-tetraacetylchitotetraose is is hydrolyzed mainly to N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose with much less cleavage into GlcNAc + N,N',N'',N'''-tetraacetylchitotetraose | Struthio camelus | N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose | - |
? | |
peptidoglycan + H2O | lytic activity against Micrococcus lysodeikticus | Struthio camelus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
goose type lysozyme | - |
Struthio camelus |
OEL | - |
Struthio camelus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
Tm-value for mutant enzyme E73S is 54.5°C | Struthio camelus |
57 | - |
Tm-value for mutant E73A is 57.4°C. Tm-value for mutant E73Q is 56.6°C | Struthio camelus |
61 | - |
Tm-value for wild-type enzyme is 60.6°C | Struthio camelus |