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Literature summary for 3.2.1.156 extracted from

  • Hong, P.Y.; Iakiviak, M.; Dodd, D.; Zhang, M.; Mackie, R.I.; Cann, I.
    Two new xylanases with different substrate specificities from the human gut bacterium Bacteroides intestinalis DSM 17393 (2014), Appl. Environ. Microbiol., 80, 2084-2093.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene rex8A, the gene is located downstream of a GH3 gene (xyl3A), DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL-21 Codon-Plus(DE3) RIL Bacteroides intestinalis

Protein Variants

Protein Variants Comment Organism
D148A site-directed mutagenesis, inactive mutant Bacteroides intestinalis
D286A site-directed mutagenesis, inactive mutant Bacteroides intestinalis
E90A site-directed mutagenesis, inactive mutant Bacteroides intestinalis

Organism

Organism UniProt Comment Textmining
Bacteroides intestinalis
-
gene rex8A
-
Bacteroides intestinalis DSM 17393
-
gene rex8A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL-21 Codon-Plus(DE3) RIL by metal affinity chromatography Bacteroides intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme hydrolyzes xylotriose through xylohexaose to a mixture of xylose and xylobiose, identification by thin layer chromatography Bacteroides intestinalis ?
-
?
additional information the enzyme hydrolyzes xylotriose through xylohexaose to a mixture of xylose and xylobiose, identification by thin layer chromatography Bacteroides intestinalis DSM 17393 ?
-
?
xylohexaose + H2O
-
Bacteroides intestinalis xylose + xylobiose
-
?
xylohexaose + H2O
-
Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
?
xylopentaose + H2O
-
Bacteroides intestinalis xylose + xylobiose
-
?
xylopentaose + H2O
-
Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
?
xylotetraose + H2O
-
Bacteroides intestinalis xylose + xylobiose
-
?
xylotetraose + H2O
-
Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
?
xylotriose + H2O
-
Bacteroides intestinalis xylose + xylobiose
-
?
xylotriose + H2O
-
Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
?

Subunits

Subunits Comment Organism
More secondary and quarternary structure analysis, gel filtration and circular dichroism spectroscopy, overview Bacteroides intestinalis

Synonyms

Synonyms Comment Organism
GH8 xylanase
-
Bacteroides intestinalis
reducing-end xylose-releasing exo-oligoxylanase
-
Bacteroides intestinalis
Rex8A
-
Bacteroides intestinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacteroides intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Bacteroides intestinalis

General Information

General Information Comment Organism
additional information three conserved catalytic residues are Glu90, Asp148, and Asp286 Bacteroides intestinalis