Cloned (Comment) | Organism |
---|---|
gene rex8A, the gene is located downstream of a GH3 gene (xyl3A), DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL-21 Codon-Plus(DE3) RIL | Bacteroides intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
D148A | site-directed mutagenesis, inactive mutant | Bacteroides intestinalis |
D286A | site-directed mutagenesis, inactive mutant | Bacteroides intestinalis |
E90A | site-directed mutagenesis, inactive mutant | Bacteroides intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides intestinalis | - |
gene rex8A | - |
Bacteroides intestinalis DSM 17393 | - |
gene rex8A | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL-21 Codon-Plus(DE3) RIL by metal affinity chromatography | Bacteroides intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme hydrolyzes xylotriose through xylohexaose to a mixture of xylose and xylobiose, identification by thin layer chromatography | Bacteroides intestinalis | ? | - |
? | |
additional information | the enzyme hydrolyzes xylotriose through xylohexaose to a mixture of xylose and xylobiose, identification by thin layer chromatography | Bacteroides intestinalis DSM 17393 | ? | - |
? | |
xylohexaose + H2O | - |
Bacteroides intestinalis | xylose + xylobiose | - |
? | |
xylohexaose + H2O | - |
Bacteroides intestinalis DSM 17393 | xylose + xylobiose | - |
? | |
xylopentaose + H2O | - |
Bacteroides intestinalis | xylose + xylobiose | - |
? | |
xylopentaose + H2O | - |
Bacteroides intestinalis DSM 17393 | xylose + xylobiose | - |
? | |
xylotetraose + H2O | - |
Bacteroides intestinalis | xylose + xylobiose | - |
? | |
xylotetraose + H2O | - |
Bacteroides intestinalis DSM 17393 | xylose + xylobiose | - |
? | |
xylotriose + H2O | - |
Bacteroides intestinalis | xylose + xylobiose | - |
? | |
xylotriose + H2O | - |
Bacteroides intestinalis DSM 17393 | xylose + xylobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary and quarternary structure analysis, gel filtration and circular dichroism spectroscopy, overview | Bacteroides intestinalis |
Synonyms | Comment | Organism |
---|---|---|
GH8 xylanase | - |
Bacteroides intestinalis |
reducing-end xylose-releasing exo-oligoxylanase | - |
Bacteroides intestinalis |
Rex8A | - |
Bacteroides intestinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacteroides intestinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Bacteroides intestinalis |
General Information | Comment | Organism |
---|---|---|
additional information | three conserved catalytic residues are Glu90, Asp148, and Asp286 | Bacteroides intestinalis |