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Literature summary for 3.2.1.149 extracted from

  • Zhou, Y.; Dong, F.; Kunimasa, A.; Zhang, Y.; Cheng, S.; Lu, J.; Zhang, L.; Murata, A.; Mayer, F.; Fleischmann, P.; Watanabe, N.; Yang, Z.
    Occurrence of glycosidically conjugated 1-phenylethanol and its hydrolase beta-primeverosidase in tea (Camellia sinensis) flowers (2014), J. Agric. Food Chem., 62, 8042-8050.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned from tea flowers, recombinant inducible expression of His-tagged enzyme in Escherichia coli Camellia sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall and in the cavity area among cells Camellia sinensis 5618
-

Organism

Organism UniProt Comment Textmining
Camellia sinensis Q7X9A9 var. Jinxuan
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and desalting gel filtration Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
anther low expression level Camellia sinensis
-
flower
-
Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenylethyl beta-D-glucopyranoside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (R)-1-phenylethanol + beta-D-glucopyranose
-
?
(R)-1-phenylethyl beta-primeveroside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (R)-1-phenylethanol + beta-primeverose
-
?
(S)-1-phenylethyl beta-D-glucopyranoside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (S)-1-phenylethanol + beta-D-glucopyranose
-
?
(S)-1-phenylethyl beta-primeveroside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (S)-1-phenylethanol + beta-primeverose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Camellia sinensis