Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Yang, S.; Fu, X.; Yan, Q.; Jiang, Z.; Wang, J.
    Biochemical characterization of a novel acidic exochitinase from Rhizomucor miehei with antifungal activity (2016), J. Agric. Food Chem., 64, 461-469 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhizomucor miehei

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 66% of initial activity Rhizomucor miehei
Co2+ 1 mM, 80% of initial activity Rhizomucor miehei
Cu2+ 1 mM, 76% of initial activity Rhizomucor miehei
Hg2+ 1 mM, 34% of initial activity Rhizomucor miehei
Mg2+ 1 mM, 86% of initial activity Rhizomucor miehei
Mn2+ 1 mM, 74% of initial activity Rhizomucor miehei
Sn2+ 1 mM, 54% of initial activity Rhizomucor miehei
Zn2+ 1 mM, 85% of initial activity Rhizomucor miehei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 glycol chitin Km value 1.55 mg/mlpH 4.5, 50°C Rhizomucor miehei
additional information
-
 colloidal chitin Km value 4.02 mg/ml, pH 4.5, 50°C Rhizomucor miehei

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rhizomucor miehei
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 165% of initial activity Rhizomucor miehei
Ni2+ 1 mM, 130% of initial activity Rhizomucor miehei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
 gel filtration Rhizomucor miehei

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei A0A286JZ72
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
 substrate tri-N-acetyl-chitotriose, pH 4.5, 50°C Rhizomucor miehei
11.9
-
 substrate colloidal chitin, pH 4.5, 50°C Rhizomucor miehei
18.1
-
 substrate glycol chitin, pH 4.5, 50°C Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O
-
 Rhizomucor miehei N,N'-diacetylchitobiose + ?
-
 ?
glycol chitin + H2O
-
 Rhizomucor miehei ?
-
 ?
N,N',N''-triacetylchitotriose + 2 H2O
-
 Rhizomucor miehei 3 beta-N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44600, SDS-PAGE, 1 * 44709, calculated Rhizomucor miehei

Synonyms

Synonyms Comment Organism
Chi44
-
 Rhizomucor miehei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half-life 1209 min Rhizomucor miehei
50
-
 half-life 324 min Rhizomucor miehei
55
-
 half-life 62 min Rhizomucor miehei
60
-
 half-life 38 min Rhizomucor miehei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 colloidal chitin pH 4.5, 50°C Rhizomucor miehei
0.016
-
 glycol chitin pH 4.5, 50°C Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Rhizomucor miehei

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9
-
 Rhizomucor miehei

pI Value

Organism Comment pI Value Maximum pI Value
Rhizomucor miehei calculated
-
 5.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 colloidal chitin kcat/Km value 0.002 ml/mg/s, pH 4.5, 50°C Rhizomucor miehei
additional information
-
 glycol chitin kcat/Km value 0.010 ml/mg/s, pH 4.5, 50°C Rhizomucor miehei