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Literature summary for 3.2.1.14 extracted from

  • Li, A.N.; Yu, K.; Liu, H.Q.; Zhang, J.; Li, H.; Li, D.C.
    Two novel thermostable chitinase genes from thermophilic fungi cloning, expression and characterization (2010), Biores. Technol., 101, 5546-5551 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermochaetoides thermophila
expression in Pichia pastoris Thermoascus aurantiacus var. levisporus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus var. levisporus Q06AK1
-
-
Thermochaetoides thermophila B3GGC7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 substrate chitosan, pH 8.0, 50°C Thermochaetoides thermophila
1.9
-
 substrate powdery chitin, pH 8.0, 50°C Thermochaetoides thermophila
3.1
-
 substrate chitosan, pH 8.0, 50°C Thermoascus aurantiacus var. levisporus
4
-
 substrate powdery chitin, pH 8.0, 50°C Thermoascus aurantiacus var. levisporus
6.1
-
 substrate colloidal chitin, pH 8.0, 50°C Thermochaetoides thermophila
26.2
-
 substrate colloidal chitin, pH 8.0, 50°C Thermoascus aurantiacus var. levisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Thermochaetoides thermophila ?
-
 ?
chitosan + H2O
-
 Thermoascus aurantiacus var. levisporus ?
-
 ?
colloidal chitin + H2O
-
 Thermochaetoides thermophila N,N'-diacetylchitobiose + ?
-
 ?
colloidal chitin + H2O
-
 Thermoascus aurantiacus var. levisporus N,N'-diacetylchitobiose + ?
-
 ?
additional information no substrate: 4-nitrophenyl-N-acetyl-D-glucosamine Thermochaetoides thermophila ?
-
 ?
additional information no substrate: 4-nitrophenyl-N-acetyl-D-glucosamine Thermoascus aurantiacus var. levisporus ?
-
 ?
powdery chitin + H2O
-
 Thermochaetoides thermophila N,N'-diacetylchitobiose
-
 ?
powdery chitin + H2O
-
 Thermoascus aurantiacus var. levisporus N,N'-diacetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 47300, SDS-PAGE, x * 45100, calculated Thermochaetoides thermophila
? x * 48400, SDS-PAGE, x * 43800, calculated Thermoascus aurantiacus var. levisporus

Synonyms

Synonyms Comment Organism
CHIT1
-
 Thermochaetoides thermophila
CHIT1
-
 Thermoascus aurantiacus var. levisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Thermoascus aurantiacus var. levisporus
60
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, 95% residual activity Thermoascus aurantiacus var. levisporus
60
-
 1 h, 97% residual activity Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thermochaetoides thermophila
8
-
-
 Thermoascus aurantiacus var. levisporus