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Literature summary for 3.2.1.14 extracted from

  • Hamre, A.G.; Eide, K.B.; Wold, H.H.; Sorlie, M.
    Activation of enzymatic chitin degradation by a lytic polysaccharide monooxygenase (2015), Carbohydr. Res., 407, 166-169.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens P07254
-
-
Serratia marcescens Q54276
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chitin + H2O substrate 3 micrometer beta-chitin Serratia marcescens ?
-
 ?

Synonyms

Synonyms Comment Organism
ChiA
-
 Serratia marcescens
ChiB
-
 Serratia marcescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 beta-chitin pH 8.0, 37°C Serratia marcescens
2.2
-
 beta-chitin pH 6.1, 37°C Serratia marcescens
9.2
-
 beta-chitin presence of lytic polysaccharide monooxygenase Cbp21, pH 6.1, 37°C Serratia marcescens
11.1
-
 beta-chitin presence of lytic polysaccharide monooxygenase Cbp21, pH 8.0, 37°C Serratia marcescens
13.9
-
 beta-chitin presence of lytic polysaccharide monooxygenase Cbp21, pH 8.0, 37°C Serratia marcescens

General Information

General Information Comment Organism
physiological function in presence of lytic polysaccharide monooxygenase Cbp21, the apparent kcat values of chitinases ChiA and ChiB increase 6-9fold, while there is no effect on chitinase ChiC Serratia marcescens