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Literature summary for 3.2.1.14 extracted from

  • Kim, H.J.; Choi, H.S.; Yang, S.Y.; Kim, I.S.; Yamaguchi, T.; Sohng, J.K.; Park, S.K.; Kim, J.C.; Lee, C.H.; Gardener, B.M.; Kim, Y.C.
    Both extracellular chitinase and a new cyclic lipopeptide, chromobactomycin, contribute to the biocontrol activity of Chromobacterium sp. C61 (2014), Mol. Plant Pathol., 15, 122-132.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis strain C61 displays antifungal activities in vitro and is used successfully for the biocontrol of plant diseases under field conditions Chromobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene chi54 Chromobacterium sp.

Protein Variants

Protein Variants Comment Organism
additional information insertional inactivation of a chi54 locus in mutant C61Chi54M abolishes growth on chitin as a sole carbon source, enzyme activity is not detected Chromobacterium sp.
T218S site-directed mutagenesis, the mutant enzyme C61Chi54TS is more active C61Chi54TS than the wild-type enzyme Chromobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Chromobacterium sp.
-
-

Organism

Organism UniProt Comment Textmining
Chromobacterium sp.
-
gene chi54
-
Chromobacterium sp. C61
-
gene chi54
-

Expression

Organism Comment Expression
Chromobacterium sp. the enzyme expression is induced by chitin addition and might be under under quorum sensing control up

General Information

General Information Comment Organism
malfunction insertional inactivation of a chi54 locus in mutant C61Chi54M abolishes growth on chitin as a sole carbon source, enzyme activity is not detected Chromobacterium sp.
physiological function strain C61 displays antifungal activities in vitro. Purified chitinase inhibits spore germination of multiple pathogens. Culture filtrates of the wild-type Chromobacterium sp. C61 and the mutant C61Chi54TS, that has a higer activity than the wild-type, both produce significant in planta biocontrol activity against rice blast, tomato leaf blight and wheat leaf rust. The enzyme expression might be under under quorum sensing control Chromobacterium sp.