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Literature summary for 3.2.1.14 extracted from
- Purification of a thermostable chitinase from Bacillus cereus by chitin affinity and its application in microbial community changes in soil (2014), Bioprocess Biosyst. Eng., 37, 1201-1209.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | activates 35% at 0.5% | Bacillus cereus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | chitin and chitinolytic bacteria addition can reduce the population of fungal plant pathogens in soil and enhance the growth of plants. In this biocontrol and environmental bioremediation, communities of soil bacteria and the addition of chitinous materials play an important role | Bacillus cereus |
| environmental protection | chitin and chitinolytic bacteria addition can reduce the population of fungal plant pathogens in soil and enhance the growth of plants. In this biocontrol and environmental bioremediation, communities of soil bacteria and the addition of chitinous materials play an important role | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | slight inhibition | Bacillus cereus | |
| EDTA | slight inhibition | Bacillus cereus | |
| Mn2+ | slight inhibition | Bacillus cereus | |
| PMSF | slight inhibition | Bacillus cereus | |
| Zn2+ | slight inhibition | Bacillus cereus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight activation | Bacillus cereus | |
| additional information | no effects by Ca2+ and Fe2+, and poor effects by Tween 20, Tween 40, and Triton X-100 at 0.5-2% | Bacillus cereus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
isolated from Tamsui mangrove river sediment in Taiwan | - |
| Bacillus cereus TKU028 | - |
isolated from Tamsui mangrove river sediment in Taiwan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 11.5fold and 10.4fold, respectively, by ammonium sulfate fractionation, colloidal chitin adsorption, dialysis and ammonium sulfate precipitation, or anion exchange chromatography and ammonium sulfate precipitation | Bacillus cereus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:chitin-grown cell | with shrimp head powder as the sole carbon/nitrogen source | Bacillus cereus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.397 | - |
purified native enzyme, pH 7.0, 37°C, affinity purification method | Bacillus cereus |
| 6.463 | - |
purified native enzyme, pH 7.0, 37°C, anion exchange purification method | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-type chitin + H2O | 99% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| alpha-type chitin + H2O | 99% of the activity with colloidal chitin | Bacillus cereus TKU028 | ? | - |
? | |
| alpha-type chitosan + H2O | 98% deacetylated, 18% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| beta-type chitin + H2O | best substrate, 148% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| beta-type chitosan + H2O | 98% deacetylated, 26% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| chitosan + H2O | 60-95% deacetylated, 24-36% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| chitosan + H2O | 60-95% deacetylated, 24-36% of the activity with colloidal chitin | Bacillus cereus TKU028 | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus cereus | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus cereus TKU028 | ? | - |
? | |
| glycol chitosan + H2O | 83% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| glycol chitosan + H2O | 83% of the activity with colloidal chitin | Bacillus cereus TKU028 | ? | - |
? | |
| additional information | substrate specificity, overview. No activity with carboxymethyl cellulose | Bacillus cereus | ? | - |
? | |
| additional information | substrate specificity, overview. No activity with carboxymethyl cellulose | Bacillus cereus TKU028 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Bacillus cereus |
| More | tryptic peptide mapping and comparison with the exochitinase from Bacillus cereus ATCC14579 | Bacillus cereus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TKU028 chitinase | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | activity range, profile overview | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified native enzyme, 60 min, stable up to | Bacillus cereus |
| 70 | - |
purified native enzyme, 60 min, inactivation | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | activity range, profile overview | Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified native enzyme, 60 min, inactivation | Bacillus cereus |
| 3.5 | - |
purified native enzyme, 60 min, 30% activity remaining | Bacillus cereus |
| 4 | 9 | purified native enzyme, 60 min, completely stable | Bacillus cereus |
| 10 | - |
purified native enzyme, 60 min, 30% activity remaining | Bacillus cereus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacillus cereus | the enzyme is induced by chitin | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | chitinolytic enzymes attack chitin contained in the cell walls of plant pathogenic fungi and then impair the growth of fungi living in soil. Biodegradation of shrimp head powder by the chitinase of Bacillus cereus TKU028 is conducive to the growth of strain TKU028 and the coexisting bacteria | Bacillus cereus |
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