Protein Variants | Comment | Organism |
---|---|---|
E61Q | site-directed mutagenesis, almost inactive mutant | Gemmabryum coronatum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | a presequence of 23 amino acids is a putative signal peptide for transportation to the endoplasmic reticulum | Gemmabryum coronatum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22654 | - |
x * 25000, SDS-PAGE, x * 22654, mature enzyme, sequence calculation | Gemmabryum coronatum |
25000 | - |
x * 25000, SDS-PAGE, x * 22654, mature enzyme, sequence calculation | Gemmabryum coronatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gemmabryum coronatum | BcChi-A is optimized for shorter substrates than class II enzymes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gemmabryum coronatum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by gel filtration, hydrophobic interaction and ion exchange chromatography | Gemmabryum coronatum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | BcChi-A is optimized for shorter substrates than class II enzymes | Gemmabryum coronatum | ? | - |
? | |
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Gemmabryum coronatum | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose | a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant. BcChi-A catalyzes an anomer inversion reaction | ? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Gemmabryum coronatum | (GlcNAc)2 + (GlcNAc)3 | a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE, x * 22654, mature enzyme, sequence calculation | Gemmabryum coronatum |
More | BcChi-A is glycoside hydrolase family-19 chitinase lacking loops I, II, IV and V, and a C-terminal loop, which are present in the catalytic domain of plant class I and II chitinases | Gemmabryum coronatum |
Synonyms | Comment | Organism |
---|---|---|
Chi-A | - |
Gemmabryum coronatum |
chitinase-A | - |
Gemmabryum coronatum |
More | the enzyme is a family 19 chitinase | Gemmabryum coronatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Gemmabryum coronatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified enzyme, stable up to, unstable at 80°C and above | Gemmabryum coronatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Gemmabryum coronatum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | purified enzyme, stable between pH 6.0 and pH 11.0, but unstable below pH 5.0 and above pH 12 | Gemmabryum coronatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gemmabryum coronatum | isoelectric focusing | - |
3.5 |
General Information | Comment | Organism |
---|---|---|
evolution | BcChi-A is glycoside hydrolase family-19 chitinase lacking loops I, II, IV and V, and a C-terminal loop, which are present in the catalytic domain of plant class I and II chitinases. The phylogenetic relationships of BcChi-A and other GH19 chitinases shows that BcChi-A is closely related to GH19 chitinases from bacteria | Gemmabryum coronatum |