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Literature summary for 3.2.1.14 extracted from

  • Taira, T.; Mahoe, Y.; Kawamoto, N.; Onaga, S.; Iwasaki, H.; Ohnuma, T.; Fukamizo, T.
    Cloning and characterization of a small family 19 chitinase from moss (Bryum coronatum) (2011), Glycobiology, 21, 644-654.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E61Q site-directed mutagenesis, almost inactive mutant Gemmabryum coronatum

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information a presequence of 23 amino acids is a putative signal peptide for transportation to the endoplasmic reticulum Gemmabryum coronatum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22654
-
x * 25000, SDS-PAGE, x * 22654, mature enzyme, sequence calculation Gemmabryum coronatum
25000
-
x * 25000, SDS-PAGE, x * 22654, mature enzyme, sequence calculation Gemmabryum coronatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gemmabryum coronatum BcChi-A is optimized for shorter substrates than class II enzymes ?
-
?

Organism

Organism UniProt Comment Textmining
Gemmabryum coronatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by gel filtration, hydrophobic interaction and ion exchange chromatography Gemmabryum coronatum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information BcChi-A is optimized for shorter substrates than class II enzymes Gemmabryum coronatum ?
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
-
Gemmabryum coronatum N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant. BcChi-A catalyzes an anomer inversion reaction ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
Gemmabryum coronatum (GlcNAc)2 + (GlcNAc)3 a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE, x * 22654, mature enzyme, sequence calculation Gemmabryum coronatum
More BcChi-A is glycoside hydrolase family-19 chitinase lacking loops I, II, IV and V, and a C-terminal loop, which are present in the catalytic domain of plant class I and II chitinases Gemmabryum coronatum

Synonyms

Synonyms Comment Organism
Chi-A
-
Gemmabryum coronatum
chitinase-A
-
Gemmabryum coronatum
More the enzyme is a family 19 chitinase Gemmabryum coronatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
Gemmabryum coronatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
purified enzyme, stable up to, unstable at 80°C and above Gemmabryum coronatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Gemmabryum coronatum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 purified enzyme, stable between pH 6.0 and pH 11.0, but unstable below pH 5.0 and above pH 12 Gemmabryum coronatum

pI Value

Organism Comment pI Value Maximum pI Value
Gemmabryum coronatum isoelectric focusing
-
3.5

General Information

General Information Comment Organism
evolution BcChi-A is glycoside hydrolase family-19 chitinase lacking loops I, II, IV and V, and a C-terminal loop, which are present in the catalytic domain of plant class I and II chitinases. The phylogenetic relationships of BcChi-A and other GH19 chitinases shows that BcChi-A is closely related to GH19 chitinases from bacteria Gemmabryum coronatum