Cloned (Comment) | Organism |
---|---|
expression of N-terminally His6-tagged mature Ra-ChiC and of Ra-ChiC89252 domain in Escherichia coli as a soluble proteins | Ralstonia sp. |
Crystallization (Comment) | Organism |
---|---|
purified isolated interdomain loop Ra-ChiC89-252, hanging drop vapour diffusion method, mixing of 0.002 ml of protein solution with 0.002 ml of reservoir solution at 20°C, equilibration against reservoir solution, X-ray diffraction structure determination and analysis at 1.85-1.90 A resolution, presence of three, four or five Ra-ChiC89-252 molecules in the asymmetric unit, respectively | Ralstonia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia sp. | - |
- |
- |
Ralstonia sp. A-471 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble N-terminally His6-tagged mature Ra-ChiC and Ra-ChiC89252 from Escherichia coli by metal affinity and cation exchange chromatography | Ralstonia sp. |
Synonyms | Comment | Organism |
---|---|---|
ChiC | - |
Ralstonia sp. |