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Literature summary for 3.2.1.14 extracted from
- Kinetic characterization, expression and molecular modeling of a chitinase from the pacific white shrimp Litopenaeus vannamei (2009), J. Food Biochem., 33, 246-259.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | maximum activity at 0.45-0.6 M NaCl | Penaeus vannamei |  |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling using the human chitotriosidase structure as template, PDB 1LG1 | Penaeus vannamei |
General Stability
| General Stability | Organism |
|---|---|
| stable at NaCl concentrations of 0-1 M | Penaeus vannamei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.165 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose | pH 6.0, 25°C | Penaeus vannamei | |
| 0.174 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose | pH 5.5, 25°C, presence of 0.6 M NaCl | Penaeus vannamei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Penaeus vannamei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penaeus vannamei | Q8ITU3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penaeus vannamei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | - |
Penaeus vannamei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O | - |
Penaeus vannamei | 4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Penaeus vannamei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Penaeus vannamei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Penaeus vannamei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penaeus vannamei | isoelectric focusing | - |
4.7 |
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