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Literature summary for 3.2.1.14 extracted from

  • Di Maro, A.; Terracciano, I.; Sticco, L.; Fiandra, L.; Ruocco, M.; Corrado, G.; Parente, A.; Rao, R.
    Purification and characterization of a viral chitinase active against plant pathogens and herbivores from transgenic tobacco (2010), J. Biotechnol., 147, 1-6.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Nicotiana tabacum Autographa californica

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Autographa californica
Mg2+ inhibitory above 10 mM Autographa californica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose 20°C, pH 6.0 Autographa californica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5-30 mM, 10% increase in activity Autographa californica

Organism

Organism UniProt Comment Textmining
Autographa californica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from transgenic tobacco leaf Autographa californica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.7
-
 20°C, pH 6.0 Autographa californica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
-
 Autographa californica 4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Autographa californica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80
-
 Autographa californica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Autographa californica

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
 Autographa californica
10
-
 30% residual activity Autographa californica

General Information

General Information Comment Organism
physiological function purified ChiA enhances the permeability of the peritrophic membrane of larvae of Lepidoptera Bombyx mori and Heliothis virescens and inhibits spore germination and growth of the phytopatogenic fungus Alternaria alternata Autographa californica