Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 64% of initial activity, isoform Chi-56, 65% of initial activity, isoform Chi-64 | Massilia timonae | |
Hg2+ | 1 mM, 58% of initial activity, isoform Chi-56, 54% of initial activity, isoform Chi-64 | Massilia timonae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 117% of initial activity, isoform Chi-56, 131% of initial activity, isoform Chi-64 | Massilia timonae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration, isoform Chi-64 | Massilia timonae |
56000 | - |
gel filtration, isoform Chi-56 | Massilia timonae |
64000 | - |
x * 64000, isoform Chi-64, SDS-PAGE | Massilia timonae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Massilia timonae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | isolated from crab shell, 18% of the activity with colloidal chitin for isoform Chi-56, 27% for isoform Chi-64 | Massilia timonae | ? | - |
? | |
colloidal chitin + H2O | - |
Massilia timonae | ? | - |
? | |
additional information | no substrate: flake chitin, chitosan with degree of acetylation 96.9, carboxymethyl cellulose. No exochitinase activity for both isoform Chi-56 and Chi-64 | Massilia timonae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, isoform Chi-64, SDS-PAGE | Massilia timonae |
monomer | 1 * 56000, isoform Chi-560, SDS-PAGE | Massilia timonae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
isoform Chi-56 | Massilia timonae |
60 | - |
isoform Chi-64 | Massilia timonae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
both isoform Chi-56 and Chi-64 | Massilia timonae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Massilia timonae | isoelectric focusing, both isoform Chi-56 and Chi-64 | - |
8.5 |