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Literature summary for 3.2.1.14 extracted from

  • Adrangi, S.; Faramarzi, M.A.; Shahverdi, A.R.; Sepehrizadeh, Z.
    Purification and characterization of two extracellular endochitinases from Massilia timonae (2010), Carbohydr. Res., 345, 402-407.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 64% of initial activity, isoform Chi-56, 65% of initial activity, isoform Chi-64 Massilia timonae
Hg2+ 1 mM, 58% of initial activity, isoform Chi-56, 54% of initial activity, isoform Chi-64 Massilia timonae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 117% of initial activity, isoform Chi-56, 131% of initial activity, isoform Chi-64 Massilia timonae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration, isoform Chi-64 Massilia timonae
56000
-
gel filtration, isoform Chi-56 Massilia timonae
64000
-
x * 64000, isoform Chi-64, SDS-PAGE Massilia timonae

Organism

Organism UniProt Comment Textmining
Massilia timonae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O isolated from crab shell, 18% of the activity with colloidal chitin for isoform Chi-56, 27% for isoform Chi-64 Massilia timonae ?
-
?
colloidal chitin + H2O
-
Massilia timonae ?
-
?
additional information no substrate: flake chitin, chitosan with degree of acetylation 96.9, carboxymethyl cellulose. No exochitinase activity for both isoform Chi-56 and Chi-64 Massilia timonae ?
-
?

Subunits

Subunits Comment Organism
? x * 64000, isoform Chi-64, SDS-PAGE Massilia timonae
monomer 1 * 56000, isoform Chi-560, SDS-PAGE Massilia timonae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
isoform Chi-56 Massilia timonae
60
-
isoform Chi-64 Massilia timonae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
both isoform Chi-56 and Chi-64 Massilia timonae

pI Value

Organism Comment pI Value Maximum pI Value
Massilia timonae isoelectric focusing, both isoform Chi-56 and Chi-64
-
8.5