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Literature summary for 3.2.1.14 extracted from

  • Neeraja, C.; Moerschbacher, B.; Podile, A.R.
    Fusion of cellulose binding domain to the catalytic domain improves the activity and conformational stability of chitinase in Bacillus licheniformis DSM13 (2010), Biores. Technol., 101, 3635-3641.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus licheniformis

Protein Variants

Protein Variants Comment Organism
additional information construction of a deletion mutant lacking the C-terminal chitin binding domain and of a hybrid chitinase consisting of the N-terminal catalytic domain fused to cellulose binding domain from the cellobiohydrolase II gene of Trichoderma reesei. Both recombinant chitinases retain their ability to bind to glycol-chitin. The deletion mutant is more effective on colloidal chitin than the chimera. The fusion to the cdellulose binding domain improves the affinity to colloidal chitin, activity and conformational stability in the chimera when compared with the deletion mutant Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
 strain DSM13
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Bacillus licheniformis DSM 13
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 strain DSM13
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O
-
 Bacillus licheniformis chitobiose + ? enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose ?
chitohexaose + H2O
-
 Bacillus licheniformis DSM 13 chitobiose + ? enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose ?
chitotetraose + H2O
-
 Bacillus licheniformis 2 chitobiose enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose ?
chitotetraose + H2O
-
 Bacillus licheniformis DSM 13 2 chitobiose enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose ?