Application | Comment | Organism |
---|---|---|
analysis | transglycosylation reaction takes place under the conditions used for HPLC analysis, but not under the ESI-MS conditions | Cycas revoluta |
Protein Variants | Comment | Organism |
---|---|---|
F166A | decrease in specific activity to 5-10% of wild-type, the sugar residue affinity of +1 subsite is strongly reduced. No effect on IC50 value of inhibitor allosamidin | Cycas revoluta |
W197A | decrease in specific activity to 5-10% of wild-type, the sugar residue affinity of +1 subsite is strongly reduced. No effect on IC50 value of inhibitor allosamidin | Cycas revoluta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
allosamidin | - |
Cycas revoluta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cycas revoluta | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Cycas revoluta | 2 GlcNACbeta(1-4)GlcNAcbeta | some (GlcNAc)3 is produced through transglycosylation reaction | ? | |
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Cycas revoluta | GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta | - |
? | |
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Cycas revoluta | GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta | product distribution is (GlcNAc)3 >(GlcNAc)4 > (GlcNAc)2 for the wild type enzyme. Some (GlcNAc)5 is produced, without formation of GlcNAc, suggesting that it is not directly produced from the initial substrate (GlcNAc)6 but produced through transglycosylation reaction | ? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00057 | - |
wild-type, pH 5.2 | Cycas revoluta | allosamidin | |
0.0006 | - |
mutant F166A, pH 5.2 | Cycas revoluta | allosamidin | |
0.00091 | - |
mutant W197A, pH 5.2 | Cycas revoluta | allosamidin |