Any feedback?
Literature summary for 3.2.1.14 extracted from
- Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine wastes by these enzymes (2009), Mar. Biotechnol., 11, 334-344.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20% inhibition at 5 mM | Bacillus cereus |  |
| Cu2+ | 27% inhibition at 5 mM | Bacillus cereus | |
| EDTA | complete inhibition at 5 mM | Bacillus cereus | |
| Fe2+ | complete inhibition | Bacillus cereus | |
| Mg2+ | 24% inhibition at 5 mM | Bacillus cereus | |
| Mn2+ | complete inhibition | Bacillus cereus | |
| additional information | no inhibition by Zn2+ at 5 mM | Bacillus cereus | |
| PMSF | - |
Bacillus cereus | |
| SDS | 4% inhibition at 1 mM | Bacillus cereus | |
| Triton X-100 | 29% inhibition at 2% | Bacillus cereus | |
| Tween 20 | 40% inhibition at 2% | Bacillus cereus | |
| Tween 40 | 40% inhibition at 2% | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
isolated from the soil in Taiwan | - |
| Bacillus cereus TKU006 | - |
isolated from the soil in Taiwan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation and anion exchange chromatography | Bacillus cereus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimzation of culture comditions for enzyme production, overview | Bacillus cereus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.62 | - |
purified enzyme | Bacillus cereus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | 60 min, completely stable | Bacillus cereus |
| 60 | - |
60 min, 50% remaining activity | Bacillus cereus |
| 80 | - |
60 min, inactivation | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | stable | Bacillus cereus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
