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Literature summary for 3.2.1.14 extracted from
- A thermostable chitinase with chitin-binding activity from Phaseolus limensis (2008), J. Food Sci., 73, C452-C457.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Phaseolus lunatus |  |
| Cu2+ | at 2 mM | Phaseolus lunatus | |
| Mn2+ | slightly inhibiting at 1-2 mM | Phaseolus lunatus | |
| Zn2+ | slightly inhibiting at 1-2 mM | Phaseolus lunatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slightly activating | Phaseolus lunatus | |
| KCl | slightly activating at 1-2 mM | Phaseolus lunatus | |
| additional information | the enzyme does not require any metal ions for its activity, no effects on enzyme activity by Mg2+ | Phaseolus lunatus | |
| NaCl | slightly activating at 1-2 mM | Phaseolus lunatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28600 | - |
native PAGE | Phaseolus lunatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Phaseolus lunatus | - |
? | - |
? | |
| additional information | Phaseolus lunatus | the enzyme exerts a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus lunatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 108fold from dry seeds by ammonium sulfate fractionation, affinity and cation exchange chromatography | Phaseolus lunatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | dry large lima bean seeds | Phaseolus lunatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.6 | - |
purified native enzyme | Phaseolus lunatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Phaseolus lunatus | ? | - |
? | |
| colloidal chitin + H2O | - |
Phaseolus lunatus | ? | - |
? | |
| additional information | the enzyme exerts a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii | Phaseolus lunatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28600, SDS-PAGE | Phaseolus lunatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
Phaseolus lunatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | temperature profile, overview | Phaseolus lunatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable up to | Phaseolus lunatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Phaseolus lunatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH profile, overview | Phaseolus lunatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phaseolus lunatus | isoelectric focusing | - |
7.8 |
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Release 2023.1 (January 2023)
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