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Literature summary for 3.2.1.14 extracted from

  • Yan, R.; Hou, J.; Ding, D.; Guan, W.; Wang, C.; Wu, Z.; Li, M.
    In vitro antifungal activity and mechanism of action of chitinase against four plant pathogenic fungi (2008), J. Basic Microbiol., 48, 293-301.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in Escherichia coli strain DH5alpha, expression in Pichia pastoris strain GS115 Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Oryza sativa chitin from fungal cell wall, the chitinase exhibits different antifungal activities against the four fungi, which is directly correlated to the surface microstructure and the proportion of chitin in the fungal cell wall, antifungal mechanism of action of chitinase against four plant pathogenic fungi, i.e. Rhizopus stolonifer, Botrytis squamosa, Pythium aphanidermatum, and Aspergillus niger, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa P24626
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 6fold from Pichia pastoris Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 purified recombinant enzyme, and antifungal activities, overview Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
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 Oryza sativa ?
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 ?
chitin + H2O chitin from fungal cell wall, the chitinase exhibits different antifungal activities against the four fungi, which is directly correlated to the surface microstructure and the proportion of chitin in the fungal cell wall, antifungal mechanism of action of chitinase against four plant pathogenic fungi, i.e. Rhizopus stolonifer, Botrytis squamosa, Pythium aphanidermatum, and Aspergillus niger, overview Oryza sativa ?
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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 in vivo assay at Oryza sativa