Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Vaaje-Kolstad, G.; Bunaes, A.C.; Mathiesen, G.; Eijsink, V.G.
    The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of alpha- and beta-chitin (2009), FEBS J., 276, 2402-2415.
    View publication on PubMed
Search for more literature for 3.2.1.14

Activating Compound

Activating Compound Comment Organism Structure
LlCBP33A a family 33 chitin-binding protein, binds to both alpha- and beta-chitin. LlCBP33A increases the hydrolytic efficiency of chitinase LlChi18A to both alpha- and beta-chitin Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
gene LlChi18A, DNA and amino acid sequence determination and analysis, expression of the soluble His-tagged enzyme in Escherichia coli BL21(DE3) Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-chitin + H2O Lactococcus lactis
-
 ?
-
 ?
beta-chitin + H2O Lactococcus lactis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis Q9CE95 ssp. lactis, strain IL1403, a derivative of the strain IL594 that is isolated from a cheese starter culture, gene LlChi18A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged LlChi18A from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cleavage of the tag through factor Xa to a purity of over 95% Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
-
 Lactococcus lactis 4-methylumbelliferone + N,N'-diacetylchitobiose
-
 ?
alpha-chitin + H2O
-
 Lactococcus lactis ?
-
 ?
beta-chitin + H2O
-
 Lactococcus lactis ?
-
 ?
additional information LlChi18A has a shallow substrate-binding groove characteristic of nonprocessive endochitinases. LlChi18A is able to hydrolyse both chitin oligomers and artificial substrates, with no sign of processivity. Substrate specificity and binding preferences, overview Lactococcus lactis ?
-
 ?

Synonyms

Synonyms Comment Organism
LlChi18A
-
 Lactococcus lactis
More th enzyme belongs to the family 18 of glycohydrolases Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
-
 Lactococcus lactis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.4
-
 purified recombinant enzyme, stable Lactococcus lactis
5
-
 and above, purified recombinant enzyme, stable for at least 1 week Lactococcus lactis