Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits CHT1 | Pseudomonas sp. | |
Fe2+ | complete inhibition of CHT1 | Pseudomonas sp. | |
Mn2+ | complete inhibition of CHT1 | Pseudomonas sp. | |
SDS | partial inhibition at 2 mM | Pseudomonas sp. | |
Tween-20 | 21% inhibition at 2% | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. TKU015 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 6fold from strain TKU015 by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain TKU015 grows with shrimp shell wastes as the sole carbon and nitrogen source | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0069 | - |
purified CHT1, substrate chitin | Pseudomonas sp. |
0.0229 | - |
purified CHT1, substrate colloidal chitin | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. | ? | - |
? | |
chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. TKU015 | ? | - |
? | |
colloidal chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. | ? | - |
? | |
colloidal chitin + H2O | CHT1 is specific for chitin substrates | Pseudomonas sp. TKU015 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, CHT1, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
CHT1 | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
CHT1 | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable below, purified CHT1 | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
CHT1 | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | purified CHT1 | Pseudomonas sp. |