Cloned (Comment) | Organism |
---|---|
chitinase A, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Rosetta-gami B (DE 3), and coexpression of chitinase A with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium in which chitinase A dominates alpha1,3-glucanase | Streptomyces cyaneus |
chitinase I, coexpression of chitinase I with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium | Niallia circulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28789 | - |
x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation | Streptomyces cyaneus |
29000 | - |
x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation | Streptomyces cyaneus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | - |
- |
- |
Niallia circulans KA-304 | - |
- |
- |
Streptomyces cyaneus | B3XZQ2 | - |
- |
Streptomyces cyaneus SP-27 | B3XZQ2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant chitinase A 46fold from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another anion exchange chromatographical step | Streptomyces cyaneus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
purified recombinant enzyme | Streptomyces cyaneus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | colloidal or powdered substrate | Streptomyces cyaneus | ? | - |
? | |
chitin + H2O | powdered substrate | Niallia circulans | ? | - |
? | |
chitin + H2O | colloidal or powdered substrate | Streptomyces cyaneus SP-27 | ? | - |
? | |
chitin + H2O | powdered substrate | Niallia circulans KA-304 | ? | - |
? | |
ethylene glycol chitin + H2O | preferred substrate | Streptomyces cyaneus | ? | - |
? | |
ethylene glycol chitin + H2O | preferred substrate | Streptomyces cyaneus SP-27 | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-GlcNAc | Streptomyces cyaneus | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-GlcNAc | Streptomyces cyaneus SP-27 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation | Streptomyces cyaneus |
Synonyms | Comment | Organism |
---|---|---|
chitinase A | - |
Streptomyces cyaneus |
chitinase I | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces cyaneus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Streptomyces cyaneus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces cyaneus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | - |
Streptomyces cyaneus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Streptomyces cyaneus |