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Literature summary for 3.2.1.14 extracted from

  • Yano, S.; Honda, A.; Rattanakit, N.; Noda, Y.; Wakayama, M.; Plikomol, A.; Tachiki, T.
    Cloning and expression of chitinase A gene from Streptomyces cyaneus SP-27: the enzyme participates in protoplast formation of Schizophyllum commune (2008), Biosci. Biotechnol. Biochem., 72, 1853-1859.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
chitinase A, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Rosetta-gami B (DE 3), and coexpression of chitinase A with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium in which chitinase A dominates alpha1,3-glucanase Streptomyces cyaneus
chitinase I, coexpression of chitinase I with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28789
-
x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation Streptomyces cyaneus
29000
-
x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation Streptomyces cyaneus

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans KA-304
-
-
-
Streptomyces cyaneus B3XZQ2
-
-
Streptomyces cyaneus SP-27 B3XZQ2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant chitinase A 46fold from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another anion exchange chromatographical step Streptomyces cyaneus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
purified recombinant enzyme Streptomyces cyaneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal or powdered substrate Streptomyces cyaneus ?
-
?
chitin + H2O powdered substrate Niallia circulans ?
-
?
chitin + H2O colloidal or powdered substrate Streptomyces cyaneus SP-27 ?
-
?
chitin + H2O powdered substrate Niallia circulans KA-304 ?
-
?
ethylene glycol chitin + H2O preferred substrate Streptomyces cyaneus ?
-
?
ethylene glycol chitin + H2O preferred substrate Streptomyces cyaneus SP-27 ?
-
?
additional information no activity with 4-nitrophenyl-GlcNAc Streptomyces cyaneus ?
-
?
additional information no activity with 4-nitrophenyl-GlcNAc Streptomyces cyaneus SP-27 ?
-
?

Subunits

Subunits Comment Organism
? x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation Streptomyces cyaneus

Synonyms

Synonyms Comment Organism
chitinase A
-
Streptomyces cyaneus
chitinase I
-
Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Streptomyces cyaneus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
Streptomyces cyaneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Streptomyces cyaneus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9
-
Streptomyces cyaneus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5
-
Streptomyces cyaneus