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Literature summary for 3.2.1.14 extracted from

  • Zees, A.C.; Pyrpassopoulos, S.; Vorgias, C.E.
    Insights into the role of the (alpha+beta) insertion in the TIM-barrel catalytic domain, regarding the stability and the enzymatic activity of chitinase A from Serratia marcescens (2009), Biochim. Biophys. Acta, 1794, 23-31.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal stucture analysis, overview Serratia marcescens

Protein Variants

Protein Variants Comment Organism
additional information an alpha + beta insertion between the B7 beta-strand and A7 alpha-helix of the TIM-barrel, an FnIII domain at the N-terminus of the molecule and a hinge region that connects the latter to the catalytic domain. Structure modelling of the (alpha + beta)DELTAChiA mutant, overview Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and alpha/beta domain deletion mutant, overview Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens P07254
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O
-
Serratia marcescens ?
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Serratia marcescens ?
-
?
chitin + H2O
-
Serratia marcescens ?
-
?
additional information the enzyme is also active with chitooligosachharides from GlnNAc to GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc, overview Serratia marcescens ?
-
?

Subunits

Subunits Comment Organism
More the catalytic domain has a TIM-barrel fold, structure modelling, overview Serratia marcescens

Synonyms

Synonyms Comment Organism
ChiA
-
Serratia marcescens
chitinase A
-
Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 45 depending on the pH, overview Serratia marcescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45
-
Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
ChiA exhibits an endothermic irreversible transition at an apparent Tm of 60.7°C Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
at 58°C Serratia marcescens
6.5
-
at 42°C Serratia marcescens

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
Serratia marcescens