Crystallization (Comment) | Organism |
---|---|
crystal stucture analysis, overview | Serratia marcescens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an alpha + beta insertion between the B7 beta-strand and A7 alpha-helix of the TIM-barrel, an FnIII domain at the N-terminus of the molecule and a hinge region that connects the latter to the catalytic domain. Structure modelling of the (alpha + beta)DELTAChiA mutant, overview | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and alpha/beta domain deletion mutant, overview | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | P07254 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O | - |
Serratia marcescens | ? | - |
? | |
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Serratia marcescens | ? | - |
? | |
chitin + H2O | - |
Serratia marcescens | ? | - |
? | |
additional information | the enzyme is also active with chitooligosachharides from GlnNAc to GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc, overview | Serratia marcescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the catalytic domain has a TIM-barrel fold, structure modelling, overview | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
ChiA | - |
Serratia marcescens |
chitinase A | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | depending on the pH, overview | Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | - |
Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
ChiA exhibits an endothermic irreversible transition at an apparent Tm of 60.7°C | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
at 58°C | Serratia marcescens |
6.5 | - |
at 42°C | Serratia marcescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Serratia marcescens |