Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Rattanakit, N.; Yano, S.; Plikomol, A.; Wakayama, M.; Tachiki, T.
    Purification of Aspergillus sp. S1-13 chitinases and their role in saccharification of chitin in mash of solid-state culture with shellfish waste (2007), J. Biosci. Bioeng., 103, 535-541.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
p-nitrophenyl-GlcNAc 10 mM, 50% inhibition Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 45000, endochitinase-1, SDS-PAGE Aspergillus sp.
51000
-
 1 * 51000, endochitinase-2, SDS-PAGE Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
 S1-13
-
Aspergillus sp. S1-13
-
 S1-13
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
-
 Aspergillus sp.
8
-
-
 Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O
-
 Aspergillus sp. ?
-
 ?
colloidal chitin + H2O
-
 Aspergillus sp. S1-13 ?
-
 ?
ethylene glycol chitin + H2O low activity Aspergillus sp. ?
-
 ?
ethylene glycol chitin + H2O low activity Aspergillus sp. S1-13 ?
-
 ?
lactic acid-treated crab shell + H2O 13% of the activity with colloidal chitin Aspergillus sp. ?
-
 ?
lactic acid-treated crab shell + H2O 18% of the activity with colloidal chitin Aspergillus sp. ?
-
 ?
additional information endochitinase-1 is inert to N,N',N''-triacetylchitotriose, N,N'-diacetylchitobiose and p-nitrophenyl-GlcNAc Aspergillus sp. ?
-
 ?
additional information endochitinase-2 is not active towards ethylene glycol chitin, low activity towards N-acetylchitoologosaccharides, no hydrolysis og N,N'-diacetylchitobiose Aspergillus sp. ?
-
 ?
additional information endochitinase-1 is inert to N,N',N''-triacetylchitotriose, N,N'-diacetylchitobiose and p-nitrophenyl-GlcNAc Aspergillus sp. S1-13 ?
-
 ?
additional information endochitinase-2 is not active towards ethylene glycol chitin, low activity towards N-acetylchitoologosaccharides, no hydrolysis og N,N'-diacetylchitobiose Aspergillus sp. S1-13 ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Aspergillus sp. N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Aspergillus sp. N,N'-diacetylchitobiose + GlcNAc
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Aspergillus sp. 2 N,N'-diacetylchitobiose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Aspergillus sp. S1-13 2 N,N'-diacetylchitobiose
-
 ?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
-
 Aspergillus sp. N,N'-diacetylchitobiose + GlcNAc
-
 ?
powder chitin + H2O
-
 Aspergillus sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, endochitinase-1, SDS-PAGE Aspergillus sp.
monomer 1 * 51000, endochitinase-2, SDS-PAGE Aspergillus sp.

Synonyms

Synonyms Comment Organism
endochitinase-1
-
 Aspergillus sp.
endochitinase-2
-
 Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 endochitinase-1 Aspergillus sp.
45
-
 endochitinase-2 Aspergillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1 50 pH 5.5, 10 min, endochitinase-1, stable Aspergillus sp.
1 50 pH 5.5, 10 min, endochitinase-2, stable Aspergillus sp.
60
-
 pH 5.5, 10 min, endochitinase-1, 50% loss of activity Aspergillus sp.
70
-
 pH 5.5, 10 min, endochitinase-2, 40% loss of activity Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 endochitinase-1 Aspergillus sp.
5 5.5 endochitinase-2 Aspergillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 stable, endochitinase-1 Aspergillus sp.
4.5 6.5 stable, endochitinase-2 Aspergillus sp.