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Literature summary for 3.2.1.14 extracted from

  • Jacobson, R.L.; Studentsky, L.; Schlein, Y.
    Glycolytic and chitinolytic activities of Phlebotomus papatasi (Diptera: Psychodidae) from diverse ecological habitats (2007), Folia Parasitol., 54, 301-309.
    View publication on PubMed
Search for more literature for 3.2.1.14

Application

Application Comment Organism
analysis comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major Phlebotomus papatasi

Inhibitors

Inhibitors Comment Organism Structure
allosamidin relative inhibition of 18.1%, six concentrations from 1 to 10 microM tested Phlebotomus papatasi
erythritol relative inhibition of 18.1%, three concentrations of 10, 50, and 100 mM tested Phlebotomus papatasi
N-acetylgalactosamine 76.2% relative inhibition, three concentrations of 10, 50, and 100 mM tested Phlebotomus papatasi
N-acetylglucosamine 78.0% relative inhibition, three concentrations of 10, 50, and 100 mM tested Phlebotomus papatasi

Organism

Organism UniProt Comment Textmining
Phlebotomus papatasi
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 desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
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Source Tissue

Source Tissue Comment Organism Textmining
whole body whole fly homogenates Phlebotomus papatasi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay described, activity measured in whole body homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, no measurable activity by using the substrate 4-nitrophenyl-beta-N,N'-diacetylchitobioside, chitinase activity without any induction by blood- or sugar-meals observed Phlebotomus papatasi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O substrate concentration of 500 microM in citrate buffer Phlebotomus papatasi 4-methylumbelliferone + N,N'-diacetylchitobiose
-
 ?
additional information 4-nitrophenyl-beta-N,N'-diacetylchitobioside shows no measurable activity in whole fly homogenates, substrate concentration of 22 mM in acetate phospate buffer used Phlebotomus papatasi ?
-
 ?

Synonyms

Synonyms Comment Organism
chitinase
-
 Phlebotomus papatasi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 assay at, pH-optimum of 4.83 estimated for Phlebotomus papatasi