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Literature summary for 3.2.1.14 extracted from

  • Yano, S.; Rattanakit, N.; Honda, A.; Noda, Y.; Wakayama, M.; Plikomol, A.; Tachiki, T.
    Purification and characterization of chitinase A of Streptomyces cyaneus SP-27: an enzyme participates in protoplast formation from Schizophyllum commune mycelia (2008), Biosci. Biotechnol. Biochem., 72, 54-61.
    View publication on PubMed
Search for more literature for 3.2.1.14

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Streptomyces cyaneus
29000
-
 1 * 29000, SDS-PAGE Streptomyces cyaneus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Streptomyces cyaneus colloidal chitin: 30% of the activity with ethylene glycol chitin, powdered chitin: 4.5% of the activity with ethylene glycol chitin ?
-
 ?
chitin + H2O Streptomyces cyaneus SP-27 colloidal chitin: 30% of the activity with ethylene glycol chitin, powdered chitin: 4.5% of the activity with ethylene glycol chitin ?
-
 ?
ethylene glycol chitin + H2O Streptomyces cyaneus
-
 ?
-
 ?
ethylene glycol chitin + H2O Streptomyces cyaneus SP-27
-
 ?
-
 ?
additional information Streptomyces cyaneus chitinase A is an endo-type enzyme. Chitinase A participates in protoplast formation from Schizophyllum commune mycelia. No activity with N,N'-diacetylchitobiose, N,N',N''-triacetylchitotriose and p-nitrophenyl-beta-D-N-acetylglucosaminide ?
-
 ?
additional information Streptomyces cyaneus SP-27 chitinase A is an endo-type enzyme. Chitinase A participates in protoplast formation from Schizophyllum commune mycelia. No activity with N,N'-diacetylchitobiose, N,N',N''-triacetylchitotriose and p-nitrophenyl-beta-D-N-acetylglucosaminide ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O Streptomyces cyaneus
-
 N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O Streptomyces cyaneus SP-27
-
 N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O Streptomyces cyaneus
-
 2 N,N'-diacetylchitobiose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O Streptomyces cyaneus SP-27
-
 2 N,N'-diacetylchitobiose
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces cyaneus
-
-
-
Streptomyces cyaneus SP-27
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces cyaneus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Streptomyces cyaneus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.4
-
-
 Streptomyces cyaneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal chitin: 30% of the activity with ethylene glycol chitin, powdered chitin: 4.5% of the activity with ethylene glycol chitin Streptomyces cyaneus ?
-
 ?
chitin + H2O colloidal chitin: 30% of the activity with ethylene glycol chitin, powdered chitin: 4.5% of the activity with ethylene glycol chitin Streptomyces cyaneus SP-27 ?
-
 ?
ethylene glycol chitin + H2O
-
 Streptomyces cyaneus ?
-
 ?
ethylene glycol chitin + H2O
-
 Streptomyces cyaneus SP-27 ?
-
 ?
additional information chitinase A is an endo-type enzyme. Chitinase A participates in protoplast formation from Schizophyllum commune mycelia. No activity with N,N'-diacetylchitobiose, N,N',N''-triacetylchitotriose and p-nitrophenyl-beta-D-N-acetylglucosaminide Streptomyces cyaneus ?
-
 ?
additional information chitinase A is an endo-type enzyme. Chitinase A participates in protoplast formation from Schizophyllum commune mycelia. No activity with N,N'-diacetylchitobiose, N,N',N''-triacetylchitotriose and p-nitrophenyl-beta-D-N-acetylglucosaminide Streptomyces cyaneus SP-27 ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Streptomyces cyaneus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Streptomyces cyaneus SP-27 N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Streptomyces cyaneus 2 N,N'-diacetylchitobiose
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Streptomyces cyaneus SP-27 2 N,N'-diacetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Streptomyces cyaneus

Synonyms

Synonyms Comment Organism
chitinase A
-
 Streptomyces cyaneus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 6.5, 10 min, stable Streptomyces cyaneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptomyces cyaneus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 stable pH-range Streptomyces cyaneus