KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for glycolchitin is 0.182 mg/ml | Scomber japonicus | |
additional information | - |
additional information | Km-value for glycolchitin is 0.471 mg/ml, isoenzyme HoChiB | Hexagrammos otakii | |
additional information | - |
additional information | Km-value for glycolchitin is 0.494 mg/ml, isoenzyme HoChiC | Hexagrammos otakii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, isoenzyme HoChiC, SDS-PAGE | Hexagrammos otakii |
51000 | - |
x * 51000, isoenzyme HoChiB, SDS-PAGE | Hexagrammos otakii |
62000 | - |
x * 62000, isoenzyme HoChiA, SDS-PAGE | Hexagrammos otakii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hexagrammos otakii | - |
- |
- |
Manduca sexta | - |
- |
- |
Scomber japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme HoChiA | Hexagrammos otakii |
isoenzyme HoChiB | Hexagrammos otakii |
isoenzyme HoChiC | Hexagrammos otakii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | isoenzyme HoChiA | Hexagrammos otakii | - |
stomach | isoenzyme HoChiB | Hexagrammos otakii | - |
stomach | isoenzyme HoChiC | Hexagrammos otakii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.25 | - |
isoenzyme HoChiB | Hexagrammos otakii |
1.25 | - |
isoenzyme HoChiC | Hexagrammos otakii |
3.04 | - |
isoenzyme HoChiA | Hexagrammos otakii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Scomber japonicus | ? | - |
? | |
chitin + H2O | - |
Manduca sexta | ? | - |
? | |
chitin + H2O | alpha-chitin and beta-chitin, isoenzyme HoChiA | Hexagrammos otakii | ? | - |
? | |
chitin + H2O | alpha-chitin and beta-chitin, isoenzyme HoChiB | Hexagrammos otakii | ? | - |
? | |
chitin + H2O | alpha-chitin and beta-chitin, isoenzyme HoChiC | Hexagrammos otakii | ? | - |
? | |
glycolchitin + H2O | - |
Scomber japonicus | ? | - |
? | |
glycolchitin + H2O | isoenzyme HoChiA | Hexagrammos otakii | ? | - |
? | |
glycolchitin + H2O | isoenzyme HoChiB | Hexagrammos otakii | ? | - |
? | |
glycolchitin + H2O | isoenzyme HoChiC | Hexagrammos otakii | ? | - |
? | |
additional information | no activity with p-nitrophenyl-(GlcNAc) | Scomber japonicus | ? | - |
? | |
additional information | no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi386 | Manduca sexta | ? | - |
? | |
additional information | no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi535 | Manduca sexta | ? | - |
? | |
additional information | no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiA | Hexagrammos otakii | ? | - |
? | |
additional information | no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiB | Hexagrammos otakii | ? | - |
? | |
additional information | no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiC | Hexagrammos otakii | ? | - |
? | |
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O | 13% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi535 | Manduca sexta | ? | - |
? | |
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O | 27% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi386 | Manduca sexta | ? | - |
? | |
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O | 30% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose | Scomber japonicus | ? | - |
? | |
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O | isoenzyme HoChiA | Hexagrammos otakii | ? | - |
? | |
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O | low activity, isoenzyme HoChiB | Hexagrammos otakii | ? | - |
? | |
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O | low activity, isoenzyme HoChiC | Hexagrammos otakii | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | - |
Scomber japonicus | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | enzyme form MsChi386 | Manduca sexta | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | enzyme form MsChi535 | Manduca sexta | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | isoenzyme HoChiA | Hexagrammos otakii | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | isoenzyme HoChiB | Hexagrammos otakii | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | isoenzyme HoChiC | Hexagrammos otakii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, isoenzyme HoChiC, SDS-PAGE | Hexagrammos otakii |
? | x * 51000, isoenzyme HoChiB, SDS-PAGE | Hexagrammos otakii |
? | x * 62000, isoenzyme HoChiA, SDS-PAGE | Hexagrammos otakii |
Synonyms | Comment | Organism |
---|---|---|
HoChiA | - |
Hexagrammos otakii |
HoChiB | - |
Hexagrammos otakii |
HoChiC | - |
Hexagrammos otakii |
MsChi386 | - |
Manduca sexta |
MsChi535 | - |
Manduca sexta |
SjChi | - |
Scomber japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
isoenzyme HoChiA, substrate: glycolchitin | Hexagrammos otakii |
70 | - |
isoenzyme HoChiB, substrate: glycolchitin | Hexagrammos otakii |
70 | - |
isoenzyme HoChiC, substrate: glycolchitin | Hexagrammos otakii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 30% of maximal activity, 70°C: about 60% of maximal activity, isoenzyme HoChiA, substrate: glycolchitin | Hexagrammos otakii |
50 | 80 | 50°C: about 40% of maximal activity, 80°C: about 60% of maximal activity, isoenzyme HoChiB, substrate: glycolchitin | Hexagrammos otakii |
50 | 80 | 50°C: about 40% of maximal activity, 80°C: about 70% of maximal activity, isoenzyme HoChiC, substrate: glycolchitin | Hexagrammos otakii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.06 | - |
glycolchitin | isoenzyme HoChiB | Hexagrammos otakii | |
1.85 | - |
glycolchitin | isoenzyme HoChiC | Hexagrammos otakii | |
2.21 | - |
glycolchitin | isoenzyme HoChiC | Scomber japonicus | |
3.15 | - |
glycolchitin | isoenzyme HoChiA | Hexagrammos otakii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
the enzyme has highest activity around pH 2 and exhibits a second optimum at pH 8 with 35% of the maximal activity at pH 2, isoenzyme HoChiA | Hexagrammos otakii |
8 | - |
isoenzyme HoChiB | Hexagrammos otakii |
8 | - |
isoenzyme HoChiC | Hexagrammos otakii |
8 | - |
the enzyme has highest activity around pH 2 and exhibits a second optimum at pH 8 with 35% of the maximal activity at pH 2, isoenzyme HoChiA | Hexagrammos otakii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 8 | pH 1.0: about 70% of maximal activity, pH 6.0: 15% of maximal activity, pH 8.0: about 35% of maximal activity | Hexagrammos otakii |
1 | 9 | HoChiB has more than 50% activity from pH 1 to 9, and the highest activity is at pH 8 | Hexagrammos otakii |
1 | 9 | HoChiC has more than 50% activity from pH 1 to 9, and the highest activity was at pH 8 | Hexagrammos otakii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hexagrammos otakii | isoelectric focusing, pH range of 3.5-9.5, isoenzyme HoChiA | - |
5.7 |
Hexagrammos otakii | isoelectric focusing, pH range of 3.5-9.5, isoenzyme HoChiB | - |
7.6 |
Hexagrammos otakii | isoelectric focusing, pH range of 3.59.5, isoenzyme HoChiC | - |
8.8 |