Any feedback?
Literature summary for 3.2.1.14 extracted from
- Cloning, expression, and characterization of a chitinase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9 (2006), Biosci. Biotechnol. Biochem., 70, 2437-2442.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aeromonas hydrophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91650 | - |
x * 91650, calculated from sequence | Aeromonas hydrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas hydrophila | Q25BN2 | - |
- |
| Aeromonas hydrophila SUWA-9 | Q25BN2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Aeromonas hydrophila |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | when cells are grown in the presence of colloidal chitin, a 60000 Da truncated form of ChiA, that has lost the C-terminal chitin-binding domain is secreted | Aeromonas hydrophila | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | low efficiency | Aeromonas hydrophila | ? | - |
? |  |
| carboxymethyl cellulose + H2O | low efficiency | Aeromonas hydrophila SUWA-9 | ? | - |
? | |
| chitin + H2O | colloidal and powdered | Aeromonas hydrophila | ? | - |
? | |
| chitin + H2O | colloidal and powdered | Aeromonas hydrophila SUWA-9 | ? | - |
? | |
| chitosan + H2O | - |
Aeromonas hydrophila | ? | - |
? | |
| chitosan + H2O | - |
Aeromonas hydrophila SUWA-9 | ? | - |
? | |
| glycol chitin + H2O | low efficiency | Aeromonas hydrophila | ? | - |
? | |
| glycol chitin + H2O | low efficiency | Aeromonas hydrophila SUWA-9 | ? | - |
? | |
| additional information | no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose | Aeromonas hydrophila | ? | - |
? | |
| additional information | no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose | Aeromonas hydrophila SUWA-9 | ? | - |
? | |
| p-nitrophenyl-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Aeromonas hydrophila | p-nitrophenol + N,N'-diacetylchitobiose + GlcNAc | - |
? | |
| p-nitrophenyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Aeromonas hydrophila | p-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
| p-nitrophenyl-N,N',N''-triacetylchitotriose + H2O | - |
Aeromonas hydrophila | p-nitrophenol + N,N'-diacetylchitobiose + GlcNAc | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 91650, calculated from sequence | Aeromonas hydrophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChiA | - |
Aeromonas hydrophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Aeromonas hydrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | - |
Aeromonas hydrophila |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
