Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Honda, Y.; Taniguchi, H.; Kitaoka, M.
    A reducing-end-acting chitinase from Vibrio proteolyticus belonging to glycoside hydrolase family 19 (2008), Appl. Microbiol. Biotechnol., 78, 627-634.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Vibrio proteolyticus

Crystallization (Commentary)

Crystallization (Comment) Organism
expression in Escherichia coli Vibrio proteolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 60000, SDS-PAGE Vibrio proteolyticus
60088
-
 1 * 60088, calculated from sequence Vibrio proteolyticus
74000
-
 gel filtration Vibrio proteolyticus

Organism

Organism UniProt Comment Textmining
Vibrio proteolyticus Q25BT4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Vibrio proteolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
363.2
-
 substrate: N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chitin + H2O weak activity Vibrio proteolyticus ?
-
 ?
chitin + H2O weak activity Vibrio proteolyticus ?
-
 ?
ethylene glycol chitin + H2O weak activity Vibrio proteolyticus ?
-
 ?
additional information no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose, N,N'-diacetylchitobiose and N,N',N''-triacetylchitotriose. Activity with N,N',N'',N''',N''''-pentaacetylchitopentaose is 0.1% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose Vibrio proteolyticus ?
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Vibrio proteolyticus N,N'-diacetylchitobiose + N,N',N'',N''',N''''-pentaacetylchitopentaose
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O hydrolyzed at 27.8% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose. The initial alpha-anomer ratio of N,N',N'',N''',N''''-pentaacetylchitopentaose is 59%. After the hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose, only the alpha-anomer of N,N',N''-triacetylchitotriose is found after the reaction for 1 min Vibrio proteolyticus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Vibrio proteolyticus
monomer 1 * 60088, calculated from sequence Vibrio proteolyticus

Synonyms

Synonyms Comment Organism
Chi19
-
 Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vibrio proteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
 Vibrio proteolyticus