Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Chuang, H.H.; Lin, F.P.
    New role of C-terminal 30 amino acids on the insoluble chitin hydrolysis in actively engineered chitinase from Vibrio parahaemolyticus (2007), Appl. Microbiol. Biotechnol., 76, 123-133.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant VpChiA and its C-terminal truncated G589 mutantVpChiAG589 encoded in 1881 and 1791 bp DNA fragments, respectively, are expressed in Escherichia coli using the pET-20b(+) expression system Vibrio parahaemolyticus

Protein Variants

Protein Variants Comment Organism
G598X C-terminal truncated G589 mutantVpChiAG589 shows higher temperature stability than recombinant VpChiA. kcat for 4-methylumbelliferyl-N,N'-diacetylchitobiose is 1.16fold higher than wild-type value, kcat for 4-methylumbelliferyl-N,N',N''-triacetylchitotriose is 1.8fold lower than wild-type value Vibrio parahaemolyticus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 22% inhibition of VpChiA, 15% inhibition of mutant enzyme VpChiAG589 Vibrio parahaemolyticus
Cu2+ 1 mM, 38% inhibition of VpChiA, 81% inhibition of mutant enzyme VpChiAG589 Vibrio parahaemolyticus
EDTA 10 mM, 21% inhibition of VpChiA, 17% inhibition of mutant enzyme VpChiAG589 Vibrio parahaemolyticus
Fe2+ 1 mM, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
Fe3+ 1 mM, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
SDS 5%, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
Triton X-100 5%, 31% inhibition of VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
Urea 4 mM, 96% inhibition of VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00144
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus
0.00261
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
0.00775
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
0.01172
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Organism

Organism UniProt Comment Textmining
Vibrio parahaemolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAc)7 + H2O
-
 Vibrio parahaemolyticus ?
-
 ?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
-
 Vibrio parahaemolyticus ?
-
 ?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
-
 Vibrio parahaemolyticus ?
-
 ?
alpha-chitin + H2O
-
 Vibrio parahaemolyticus ?
-
 ?

Synonyms

Synonyms Comment Organism
VpChiA
-
 Vibrio parahaemolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate: 4-methylumbelliferyl-N,N'-diacetylchitobiose or 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 25% of maximal activity, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589. 50°C: 70% of maximal activity, VpChiA. 90% of maximal activity, C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 25% loss of activity of VpChiA and 5% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus
80
-
 30 min, 55% loss of activity of VpChiA and 20% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus
90
-
 30 min, 70% loss of activity of VpChiA and 60% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
0.833
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, mutant enzyme VpChiAG589 Vibrio parahaemolyticus
13.94
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
16.16
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, mutant enzyme VpChiAG589 Vibrio parahaemolyticus
21
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
68.87
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, mutant enzyme VpChiAG589 Vibrio parahaemolyticus
110.6
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, VpChiA Vibrio parahaemolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 substrate: 4-methylumbelliferyl-N,N'-diacetylchitobiose or 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 90% of maximal activity, pH 8.0: about 60% of maximal activity, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus