Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Bonfirm, S.M.R.C.; Cruz, A.H.S.; Jesuino, R.S.A.; Ulhoa, C.J.; Molinari-Madlum, E.E.W.I.; Soares, C.M.A.; Pereira, M.
    Chitinase from Paracoccidioides brasiliensis: molecular cloning, structural, phylogenetic, expression and activity analysis (2006), FEMS Immunol. Med. Microbiol., 46, 269-283.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene cts1 from yeast cells, cDNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain XL1-Blue Paracoccidioides brasiliensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the chitinase is secreted Paracoccidioides brasiliensis
-
-

Organism

Organism UniProt Comment Textmining
Paracoccidioides brasiliensis Q5YLC0 gene cts1
-
Paracoccidioides brasiliensis Pb01 / ATCC MYA 826 Q5YLC0 gene cts1
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information in yeast and in mycelium stage Paracoccidioides brasiliensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.4
-
 enzyme from inducing medium Paracoccidioides brasiliensis
13.6
-
 enzyme from non-inducing medium Paracoccidioides brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O swollen chitin Paracoccidioides brasiliensis ?
-
 ?
chitin + H2O swollen chitin Paracoccidioides brasiliensis Pb01 / ATCC MYA 826 ?
-
 ?

Subunits

Subunits Comment Organism
More secondary structure analysis, peptide mapping Paracoccidioides brasiliensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Paracoccidioides brasiliensis