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Literature summary for 3.2.1.14 extracted from

  • Han, B.K.; Moon, J.K.; Ryu, Y.W.; Park, Y.H.; Jo, D.H.
    Purification and characterization of acidic chitinases from gizzards of broiler (Gallus gallus L.) (2000), J. Biochem. Mol. Biol., 33, 326-331.
No PubMed abstract available
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM for GAC1: 1.97 mg/ml and for GAC2: 0.42 mg/ml with chitin as substrate Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48200
-
 x * 57800, SDS-PAGE, GAC2, x * 48200, SDS-PAGE, GAC1 Gallus gallus
57800
-
 x * 57800, SDS-PAGE, GAC2, x * 48200, SDS-PAGE, GAC1 Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
688000
-
 GAC1 after phenyl-sepharose purification step Gallus gallus
756300
-
 GAC2 after phenyl-sepharose purification step Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Gallus gallus ?
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Gallus gallus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Gallus gallus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity ?

Subunits

Subunits Comment Organism
? x * 57800, SDS-PAGE, GAC2, x * 48200, SDS-PAGE, GAC1 Gallus gallus

Synonyms

Synonyms Comment Organism
GAC1
-
 Gallus gallus
GAC2
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Gallus gallus

pI Value

Organism Comment pI Value Maximum pI Value
Gallus gallus chromatofocusing, GAC1 and GAC2 4.8 4.7